Modulation of tumor cell motility by prostaglandins and inhibitors of prostaglandin synthesis.
Modulation of tumor cell motility by prostaglandins and inhibitors of prostaglandin synthesis.
复制标题
前列腺素和前列腺素合成抑制剂对肿瘤细胞运动的调节。
DOI：
10.1159/000163450
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Varani,J
中科院分区：
文献类型：
--
作者：
He,XM;Fligiel,SE;Varani,J
15-甲基-前列腺素E_1(15-M-PGE_1)是一种人工合成的稳定的前列腺素E_1类似物，对小鼠肿瘤细胞系具有抑制运动的能力。当15-M-PGE1浓度低至1µM时，12-O-十四酰佛波醇醋酸酯对随机运动和12-O-十四酰佛波醇醋酸酯刺激的运动均有抑制作用，而10µM-PGE1则抑制层粘连蛋白刺激的运动。这项研究中使用的小鼠肿瘤细胞产生了高水平的前列腺素。当用消炎痛或布洛芬处理细胞时，前列腺素水平(放射免疫法测量为前列腺素E2)降低了95%以上，而脂氧合酶产物却没有相应的增加。当吲哚美辛或布洛芬处理的细胞与对照细胞在运动性方面进行比较时，它们更活跃。这些研究表明，E系列前列腺素可以调节小鼠纤维肉瘤细胞的运动，并提示小鼠肿瘤细胞产生内源性环氧合酶代谢产物可能至少部分地调节细胞对刺激的反应性。
15-Methyl-prostaglandin E1(15-M-PGE1), a synthetic stable, prostaglandin E1analogue was examined for ability to inhibit motility in a line of murine tumor cells. Inhibition of random motility and motility stimulated by 12-O-tetradecanoyl phorbol acetate was seen at concentrations of 15-M-PGE1as low as 1 µM.Inhibition of laminin-stimulated motility was observed with 10 µM15-M-PGE1. The murine tumor cells used in this study produced high levels of prostaglandins. When the cells were treated with either indomethacin or ibuprofen, prostaglandin levels (measured as prostaglandin E2by radioimmunoassay) were reduced by greater than 95% without a corresponding increase in lipoxygenase products. When indomethacin or ibuprofen-treated cells were compared to control cells in regards to motility, they were more active. These studies show that E-series prostaglandins can modulate motility in the murine fibrosarcoma cells and suggest that the production of endogenous cyclooxygenase metabolites by the murine tumor cells may regulate, at least in part, the responsiveness of the cells to stimulation.