Localization of serum resistance-associated protein in Trypanosoma brucei rhodesiense and transgenic Trypanosoma brucei brucei.
Localization of serum resistance-associated protein in Trypanosoma brucei rhodesiense and transgenic Trypanosoma brucei brucei.
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DOI：
10.1111/cmi.12454
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发表时间：
2015-10
影响因子：
3.4
通讯作者：
Navarro M
中科院分区：
文献类型：
--
作者：
Bart JM;Cordon-Obras C;Vidal I;Reed J;Perez-Pastrana E;Cuevas L;Field MC;Carrington M;Navarro M
非洲锥虫感染广泛的哺乳动物，但人类和一些高等灵长类动物受到含有载脂蛋白l1的血清锥虫分解因子的保护。在人类感染的布氏锥虫亚种中，T. b. rhodesiense是一种源自变异表面糖蛋白基因家族成员的基因产物，血清耐药相关蛋白（SRA蛋白）可保护apol - l1介导的裂解。对锥虫裂解因子的保护需要SRA蛋白与锥虫内噬器内载脂蛋白li的直接相互作用，但这种相互作用的确切机制和位置仍存在一些不确定性。为了更深入地了解SRA介导的对锥虫溶酶因子的抗性机制，我们使用一种针对SRA的新型单克隆抗体和一组特征明确的内体标记物，评估了SRA在布氏罗得西亚锥虫EATRO3中的定位。通过3D-IF单细胞分析，结合双标记免疫电镜，我们发现约50%的SRA蛋白定位在溶酶体上，其余的SRA蛋白通过内吞途径分布，但在鞭毛袋膜上明显缺失。这些数据表明，SRA/TLF相互作用是在细胞内进行的，内体内的浓度可能对确保高效率至关重要。
African trypanosomes infect a broad range of mammals, but humans and some higher primates are protected by serum trypanosome lytic factors that contain Apo-L1. In the human infective subspecies of Trypanosoma brucei, T. b. rhodesiense, a gene product derived from the variant surface glycoprotein gene family member, serum-resistance associated protein (SRA protein) protects against Apol-L1-mediated lysis. Protection against trypanosome lytic factor requires the direct interaction between SRA protein and Apo-LI within the endocytic apparatus of the trypanosome, but some uncertainty remains as to the precise mechanism and location of this interaction. In order to provide more insight into the mechanism of SRA-mediated resistance to trypanosome lytic factor, we assessed the localisation of SRA in T.b. rhodesiense EATRO3, using a novel monoclonal antibody raised against SRA together with a set of well-characterized endosomal markers. By 3D-IF single cell analysis, combined with double-labeling immunoelectron microscopy, we found that ≈50% of SRA protein localized to the lysosome, with the remaining population being distributed through the endocytic pathway, but apparently absent from the flagellar pocket membrane. These data suggest that the SRA/TLF interaction is intracellular, with the concentration within the endosomes potentially crucial for ensuring a high efficiency.
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Brucei gambiense对不同哺乳动物血清的适应性锥虫与VSG表达位点可塑性有关。
影响因子：
3.7
作者：
Cordon-Obras C;Cano J;González-Pacanowska D;Benito A;Navarro M;Bart JM
通讯作者：
Bart JM
DOI：
10.1083/jcb.200902119
发表时间：
2009-07-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Landeira D;Bart JM;Van Tyne D;Navarro M
通讯作者：
Navarro M
影响因子：
64.5
作者：
Engstler, Markus;Pfohl, Thomas;Overath, Peter
通讯作者：
Overath, Peter
影响因子：
1.5
作者：
Bringaud, F;Robinson, DR;Baltz, T
通讯作者：
Baltz, T
影响因子：
--
作者：
Sutterwala, Shaheen S.;Creswell, Caleb H.;Bangs, James D.
通讯作者：
Bangs, James D.