Experimental test on the use of MS-222 for ostracod anaesthesia: concentration, immersion period and recovery time
Experimental test on the use of MS-222 for ostracod anaesthesia: concentration, immersion period and recovery time
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DOI：
10.4081/jlimnol.2010.350
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发表时间：
2010-01-01
影响因子：
1.6
通讯作者：
Mezquita, Francesc
中科院分区：
文献类型：
--
作者：
Schmit, Olivier;Mezquita, Francesc
动物麻醉可用于不同的目的，特别是出于兽医原因或实验研究，用于操纵或治疗固定但活着的动物。它在甲壳类动物中的应用并不罕见，但从未有关于介形类动物的描述。我们提供了关于使用甲磺酸三卡因 (MS-222) 治疗广泛分布的淡水介形类 Eucypris virens 的简要初步指南，并且我们表明该化合物是一种有效的麻醉剂，最低浓度为 500 mg L-1 时可用作浴处理。该值远高于其他水生动物（如鱼类）的推荐值。恢复时间为 5 至 15 分钟，主要取决于麻醉浴浓度，而麻醉浴持续时间影响较小。 MS-222 诱导的麻醉可用于对活介形类动物（例如介形类动物）进行精细操作。用于在显微镜下进行识别、标记或图像捕获。
Anaesthesia of animals may be useful for different purposes, particularly for veterinary reasons or in experimental research, for manipulation or treatment of immobilized but alive animals. Its use in crustaceans is not uncommon, but it has never been described for Ostracoda. We provide brief and preliminary guidelines on the use of the tricaine mesylate (MS-222) on the widespread freshwater ostracod Eucypris virens and we show that this compound is an effective anaesthetic used as a bath treatment at minimum concentrations of 500 mg L-1. This value is considerably higher than that recommended for other aquatic animals like fish. Recovery time, ranging from 5 to 15 minutes, is mostly determined by anaesthetic bath concentration, while bath duration influenced to a lesser extent. Anaesthesia induced with MS-222 can prove useful for minute manipulation of living ostracods e.g. for identification, marking or image capture under the microscope.