Changes in the organization of the lipid bilayer of the plasma membrane during spermatogenesis and epididymal maturation.
Changes in the organization of the lipid bilayer of the plasma membrane during spermatogenesis and epididymal maturation.
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DOI：
10.1095/biolreprod34.2.379
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发表时间：
1986-03
影响因子：
3.6
通讯作者：
R. Schlegel;R. Hammerstedt;G. Cofer;K. Kozarsky;D. Freidus;P. Williamson
中科院分区：
文献类型：
--
作者：
R. Schlegel;R. Hammerstedt;G. Cofer;K. Kozarsky;D. Freidus;P. Williamson
公羊、公牛和小鼠精子细胞用几种荧光膜探针染色。与非特异性探针相反，mercury 540（MC 540），它显示出优先结合模型膜中松散包装的磷脂，是专门定位于头部的前部和成熟精子的中段。为了确定在发育过程中何时获得这种独特的染色模式，分离并检查了青春期前和成年小鼠睾丸的生殖细胞以及附睾头、附睾体和附睾尾的精子。在精子到达附睾体之前，没有观察到MC 540的局部染色，在附睾体中，那些具有完全易位的细胞（即，位于远端）的细胞质液滴发出荧光。同样，当精子用荧光标记的伴刀豆球蛋白A（fl-ConA）染色时，在到达附睾体之前，没有观察到凝集素限制在头部前部的局部荧光图案。然而，与MC 540染色相反，只有一部分具有完全易位液滴的精子表现出fl-ConA的这种局部染色，其余部分在整个细胞上表现出弥漫荧光，如在附睾精子头上所见。染色模式的这些发育变化是鼠细胞特有的，因为在公羊的附睾精子上未观察到MC 540或fl-ConA染色模式的变化。这些结果进行了讨论：1）种与种之间的差异，精子膜功能;和2）的假设，即域的松散包装的脂质可能参与区域化的膜蛋白发生在精子发育过程中。
Ram, bull, and mouse sperm cells were stained with several fluorescent membrane probes. In contrast to nonspecific probes, merocyanine 540 (MC540), which displays preferential binding to loosely packed phospholipids in model membranes, was specifically localized to the anterior portion of the head and the midpiece of mature sperm. To establish when during development this distinctive staining pattern was acquired, germ cells from prepubescent and adult mouse testes as well as sperm from the caput, corpus, and cauda epididymides were isolated and examined. Localized staining with MC540 was not observed until sperm reached the corpus epididymidis, where those cells with a completely translocated (i.e., distally located) cytoplasmic droplet fluoresced. Likewise, when sperm were stained with fluoresceinated concanavalin A (fl-ConA), a localized pattern of fluorescence with lectin restricted to the anterior portion of the head was not observed until the corpus epididymidis was reached. However, in contrast to MC540 staining, only a fraction of sperm with completely translocated droplets exhibited this localized staining with fl-ConA, the remainder exhibiting diffuse fluorescence over the entire cell as seen on caput epididymal sperm. These developmental changes in staining patterns are specific to murine cells, since no change in the pattern of staining by either MC540 or fl-ConA was seen on epididymal sperm of the ram. These results are discussed with respect to: 1) species-to-species differences in sperm membrane features; and 2) the hypothesis that domains of loosely packed lipids may be involved in the regionalization of membrane proteins that occurs during sperm development.