Comparative proteome analysis of breast cancer and adjacent normal breast tissues in human.
Comparative proteome analysis of breast cancer and adjacent normal breast tissues in human.
复制标题
DOI：
10.1016/s1672-0229(06)60029-6
复制
发表时间：
2006-08
期刊：
影响因子：
--
通讯作者：
Yang HJ
中科院分区：
文献类型：
--
作者：
Deng SS;Xing TY;Zhou HY;Xiong RH;Lu YG;Wen B;Liu SQ;Yang HJ
采用二维聚丙烯酰胺凝胶电泳（2D-PAGE）和基质辅助激光解吸/电离串联飞行时间质谱（MALDI-TOF/TOF-MS），结合在线数据库检索，研究乳腺癌与邻近正常乳腺组织的差异蛋白。考虑到正常对照中血清白蛋白丰富，12例乳腺癌样本中有11例（91.7%）通过在线siene - 2dpage数据库检索和MALDI-TOF/TOF-MS分析鉴定出15个差异点。结果表明，乳腺癌的病理变化与物质代谢增强、蛋白水解活性增强、某些酶抑制剂活性下降等有关。一些在乳腺癌病理过程中表达改变的重要蛋白可能是有用的生物标志物，如α -1-抗胰蛋白酶、ef -1- β、组织蛋白酶D、TCTP、SMT3A、RPS12、PSMA1，其中SMT3A、RPS12、PSMA1在本研究中首次被报道用于乳腺癌。
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS), incorporated with online database searching, were performed to investigate differential proteins of breast cancer and adjacent normal breast tissues. Considering that serum albumin is abundantly presented in normal control samples, 15 differential spots detected in 11 out of 12 (91.7%) breast cancer samples were identified by online SIENA-2DPAGE database searching and MALDI-TOF/TOF-MS analysis. The results indicate that pathological changes of breast cancer are concerned with augmentation of substance metabolism, promotion of proteolytic activity, decline of activity of some inhibitors of enzymes, and so on. Some important proteins involved in the pathological process of breast cancer with changed expression may be useful biomarkers, such as alpha-1-antitrypsin, EF-1-beta, cathepsin D, TCTP, SMT3A, RPS12, and PSMA1, among which SMT3A, RPS12, and PSMA1 were first reported for breast cancer in this study.