Purification of the cardiac 1,4-dihydropyridine receptor/calcium channel complex.
Purification of the cardiac 1,4-dihydropyridine receptor/calcium channel complex.
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心脏 1,4-二氢吡啶受体/钙通道复合物的纯化。
DOI：
10.1016/0006-291x(85)90563-7
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发表时间：
1985
影响因子：
3.1
通讯作者：
Hosey,MM
中科院分区：
文献类型：
--
作者：
Rengasamy,A;Ptasienski,J;Hosey,MM
鸡心膜用[3H]PN 200-110（一种1,4-二氢吡啶钙通道拮抗剂）标记。 [3H]PN 200-110 受体用洋地黄皂苷溶解，并使用多种色谱系统和蔗糖密度梯度离心纯化 600 倍。凝胶电泳分析表明，最终产物富含两种分子量分别为 60,000 和 54,000 的肽，以及另一种分子量为 34,000 的肽。
Chick heart membranes were labelled with [3H]PN 200-110, a 1,4-dihydropyridine calcium channel antagonist. The [3H]PN 200-110 receptors were solubilized with digitonin and purified 600-fold using several chromatographic systems and sucrose density gradient centrifugation. Analyses by gel electrophoresis revealed that the final product was enriched in two peptides with molecular weights of 60,000 and 54,000, and another of 34,000.