Cholinesterase inhibitory effects of the extracts and compounds of Maclura pomifera (Rafin.) Schneider
Cholinesterase inhibitory effects of the extracts and compounds of Maclura pomifera (Rafin.) Schneider
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DOI：
10.1016/j.fct.2009.04.023
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发表时间：
2009-08-01
影响因子：
4.3
通讯作者：
Mokry, P.
中科院分区：
文献类型：
--
作者：
Orhan, I.;Senol, F. S.;Mokry, P.
我们已经调查了马杜拉pomifera（拉芬）的叶，木材，花，树枝，茎皮的女性和男性个体和根皮和果实的甲醇提取物的抗胆碱酯酶的潜力。Schneider（桑科）沿着其主要的生物碱; osajin和pomiferin以及它们的半合成衍生物;异osajin和异pomiferin。抗胆碱酯酶活性测定采用Ellman法，酶联免疫吸附测定仪。Osajin和pomiferin对AChE有明显的抑制作用，IC 50值分别为2.239和0.096 mM，而它们的异源衍生物对AChE的抑制作用较小。提取物和化合物不抑制BChE。通过LC-DAD-MS分析提取物的osajin和pomiferin含量，并且仅果实和雌花含有osajin（果实：8.87%，雌花：0.19%，w/w）和pomiferin（果实：13.6%，雌花：0.36%，w/w）。(C)2009爱思唯尔有限公司版权所有。
We have investigated anticholinesterase potential of the methanol extracts from the leaf, wood, flower, twig, and stem bark of the female and male individuals and rhizodermis and fruit from the female tree of Madura pomifera (Rafin.) Schneider (Moraceae) along with its major isoflavonoids; osajin and pomiferin as well as their semi-synthetic derivatives; iso-osajin and iso-pomiferin. Anticholinesterase activity was determined by Ellman method using ELISA microplate reader. Osajin and pomiferin had a noticeable inhibition of AChE with IC50 values of 2.239 and 0.096 mM, respectively, while their iso-derivatives were found to display less inhibition towards AChE. The extracts and compounds did not inhibit BChE. The extracts were analyzed for osajin and pomiferin contents by LC-DAD-MS and only the fruits and female flowers contained osajin (fruit: 8.87%, female flowers: 0.19%, w/w) and pomiferin (fruit: 13.6%, female flowers: 0.36%, w/w). (C) 2009 Elsevier Ltd. All rights reserved.