Increased pulmonary response to inhaled endotoxin in lactating rats.
Increased pulmonary response to inhaled endotoxin in lactating rats.
复制标题
DOI：
10.1164/ajrccm/147.5.1100
复制
发表时间：
1993-05
期刊：
影响因子：
--
通讯作者：
T. Gordon;P. Weideman;A. Gunnison
中科院分区：
文献类型：
--
作者：
T. Gordon;P. Weideman;A. Gunnison
风险评估的一个重要方面是确定特别容易受到吸入污染物影响的亚群。本研究旨在探讨哺乳期雌性大鼠是否对吸入内毒素所致的急性肺损伤更为敏感。将哺乳期和年龄匹配的处女大鼠暴露于生理盐水或内毒素气雾剂中3h，并于暴露后24h进行灌洗。未孕大鼠和哺乳期大鼠暴露于生理盐水后，乳酸脱氢酶、β-葡萄糖醛酸酶、总蛋白、总细胞和中性粒细胞计数均无显著差异。与暴露于29.6微克/立方米内毒素的处女大鼠相比，在哺乳期，除β-葡萄糖醛酸酶外，肺损伤的各项标志物都要高出1.5到3倍。暴露于1.3微克/立方米内毒素的哺乳期大鼠的中性粒细胞(6倍)、细胞总数和蛋白质也显著增加(p<；0.05)，据报道，这种浓度在许多农业环境中都会发生。这些结果表明，哺乳期的生理状态与对吸入内毒素引起的急性肺损伤的敏感性增加有关，并与先前的工作一致，表明对臭氧暴露的敏感性类似地增加。在相似的人类群体中出现类似的增强反应模式的可能性值得研究。
An important aspect of risk assessment is identification of subpopulations particularly susceptible to the effects of inhaled pollutants. The present study examined whether female rats were more sensitive during lactation to the acute pulmonary injury produced by inhaled endotoxin. Lactating and age-matched virgin female rats were exposed to aerosols of saline or endotoxin for 3 h and lavaged at 24 h after exposure. No significant differences in lactate dehydrogenase, beta-glucuronidase, total protein, and total cell and PMN counts were observed between virgin and lactating rats after exposure to saline. Each marker of pulmonary injury except beta-glucuronidase was 1.5- to 3-fold greater in lactating than in virgin female rats exposed to 29.6 micrograms/m3 endotoxin. PMNs (6-fold), total cell counts, and protein were also significantly increased (p < 0.05) in lactating rats exposed to 1.3 micrograms/m3 endotoxin, a concentration reported to occur in a number of agricultural settings. These results demonstrate that the physiologic state of lactation is associated with an increased sensitivity to the acute pulmonary injury produced by inhaled endotoxin and are consistent with previous work demonstrating a similar increased sensitivity to ozone exposure. The possibility of a similar pattern of enhanced response in analogous groups of humans merits examination.