Microglia Preserve Visual Function in a Mouse Model of Retinitis Pigmentosa with Rhodopsin-P23H Mutant.
Microglia Preserve Visual Function in a Mouse Model of Retinitis Pigmentosa with Rhodopsin-P23H Mutant.
复制标题
小胶质细胞在视网膜色素变性小鼠模型中保留视紫红质-P23H 突变体的视觉功能。
DOI：
10.1007/978-3-031-27681-1_62
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发表时间：
2023
影响因子：
--
通讯作者：
Saban,DanielR
中科院分区：
文献类型：
--
作者：
Yu,Chen;Saban,DanielR
大多数形式的视网膜外退行性疾病涉及视网膜下间隙小胶质细胞/巨噬细胞的异位积累，包括视网膜色素变性。然而，它们在视网膜变性过程中光感受器功能丧失中的作用尚不清楚。在这里，我们研究了条件小胶质细胞耗损对视紫红质P23H突变小鼠的光感受器数量和视觉功能的影响，视紫红质P23H突变是人类色素性视网膜炎的主要形式。我们发现小胶质细胞耗损导致视紫红质水平升高和光感受器层厚度增加。然而，视网膜的整体电生理功能随着小胶质细胞的缺失而降低。因此，这些结果确定了小胶质细胞在保护视网膜外变性的视觉功能方面的重要作用。
Most forms of outer retinal degenerative diseases involve the ectopic accumulation of microglia/macrophages in the subretinal space, including retinitis pigmentosa. However, their role in the loss of photoreceptor function during retinal degeneration remains unknown. Here, we examined the effect of conditional microglial depletion on photoreceptor numbers and visual function in mice with the rhodopsin P23H mutation, a dominant form of retinitis pigmentosa in humans. We found that microglial depletion led to an elevated level of rhodopsin and increased photoreceptor layer thickness. However, overall electrophysiological functions of the retina were reduced with microglial depletion. Therefore, these results identify an essential role of microglia specially in preserving visual function in outer retinal degeneration.