Development and Evaluation of a Perfusion Decellularization Porcine Heart Model - Generation of 3-Dimensional Myocardial Neoscaffolds
Development and Evaluation of a Perfusion Decellularization Porcine Heart Model - Generation of 3-Dimensional Myocardial Neoscaffolds
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DOI：
10.1253/circj.cj-10-0717
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发表时间：
2011-04-01
影响因子：
3.3
通讯作者：
Szabo, Gabor
中科院分区：
文献类型：
--
作者：
Weymann, Alexander;Loganathan, Sivakkanan;Szabo, Gabor
背景：关于用于心肌组织工程的三维新支架的生成的报道有限。整个心脏的灌流去细胞所提供的结构将支持从无细胞基质中生产出人类大小的三维活组织。本研究的目的是评估灌流脱细胞模型用于全心脏组织工程的可能性。方法和结果：取自12名德国长白鼠的心脏。来自选定屠宰场的猪。在准备好后，将心脏安装在专门为此目的构建的修改的朗宁多夫脱细胞模型上并进行灌流。去细胞是通过基于离子洗涤剂的灌流方案实现的。通过组织学和荧光显微镜对脱细胞过程的质量进行量化。用分光光度法定量测量脱细胞心脏中残留DNA的数据，并与对照组进行比较。经过组织学检查，所有心脏都缺乏细胞内成分，但保留了各种类型的胶原、蛋白多糖和弹性蛋白。与对照组相比，脱细胞心脏的DNA含量显著降低(84.32±3.99ngDNA/mg组织vs.470.13+/-18.77ngDNA/mg组织，P
Background: Reports about the generation of 3-dimensional neoscaffolds for myocardial tissue engineering are limited. The architecture provided by perfusion decellularization of whole hearts would support the production of human-sized 3-dimensional living tissues from an acellular matrix. The aim of this study was to evaluate the potential of a perfusion decellularization model for whole heart tissue engineering.Methods and Results: Hearts were obtained from 12 German Landrace. pigs from a selected abattoir. After preparation, the hearts were mounted and perfused on a modified Langendorff decellularization model specifically constructed for this reason. Decellularization was achieved by an ionic detergent-based perfusion protocol. The quality of the decellularization process was quantified by histology and fluorescence microscopy. Data regarding the presence of residual DNA within the decellularized hearts was measured with spectrophotometric quantification and compared to controls. After histological examination, all hearts lacked intracellular components but retained various types of collagen, proteoglycan and elastin. Quantitative DNA analysis demonstrated a significant reduction of DNA in decellularized hearts compared to controls (84.32 +/- 3.99 ng DNA/mg tissue vs. 470.13 +/- 18.77 ng DNA/mg tissue (P