Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics
Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics
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DOI：
10.1038/s41598-017-10088-5
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发表时间：
2017-08
影响因子：
4.6
通讯作者：
A. K. Schellhaus;Daniel Moreno-Andrés;M. Chugh;H. Yokoyama;Athina Moschopoulou;Suman De;F. Bono;K. Hipp;E. Schäffer;Wolfram Antonin
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作者：
A. K. Schellhaus;Daniel Moreno-Andrés;M. Chugh;H. Yokoyama;Athina Moschopoulou;Suman De;F. Bono;K. Hipp;E. Schäffer;Wolfram Antonin
有丝分裂纺锤体是将姐妹染色单体分离为两个进化的子细胞所必需的，它由高度动态的细胞骨架细丝，即微管组成。微管的动力学是由许多微管相关蛋白调控的。我们在这里确定了发育调节的GTP结合蛋白1 （DRG1）是一种微管结合蛋白，具有多种微管相关功能。在体外，DRG1可以在微管上扩散，促进微管聚合，驱动微管成束，稳定微管。DRG1水平降低的HeLa细胞从前期到后期的进展延迟，因为纺锤体形成减慢。虽然DRG1是一种GTP酶，但为了实现其微管相关功能，它不需要GTP水解。然而，所有结构域都是必需的，因为截断的版本除了微管结合外没有显示上述活性。
The mitotic spindle, essential for segregating the sister chromatids into the two evolving daughter cells, is composed of highly dynamic cytoskeletal filaments, the microtubules. The dynamics of microtubules are regulated by numerous microtubule associated proteins. We identify here Developmentally regulated GTP binding protein 1 (DRG1) as a microtubule binding protein with diverse microtubule-associated functions.In vitro, DRG1 can diffuse on microtubules, promote their polymerization, drive microtubule formation into bundles, and stabilize microtubules. HeLa cells with reduced DRG1 levels show delayed progression from prophase to anaphase because spindle formation is slowed down. To perform its microtubule-associated functions, DRG1, although being a GTPase, does not require GTP hydrolysis. However, all domains are required as truncated versions show none of the mentioned activities besides microtubule binding.