Comparison of the genotyping results using DNA obtained from blood and saliva.
Comparison of the genotyping results using DNA obtained from blood and saliva.
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DOI：
10.1097/ypg.0b013e3283060f81
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发表时间：
2008-12
影响因子：
0.9
通讯作者：
Brody GH
中科院分区：
文献类型：
--
作者：
Philibert RA;Zadorozhnyaya O;Beach SR;Brody GH
传统上，大规模基因分型项目使用来自全血或淋巴母细胞系的 DNA。但在过去几年中，许多研究人员已经开始使用从唾液中制备的 DNA 进行基因分型研究，特别是用于行为遗传学研究。然而，这两个来源的 DNA 的可比性尚未经过公正来源的严格分析。在本次通讯中，我们将来自爱荷华州收养研究的 474 名受试者的全血样本的 DNA 与从坚强非裔美国人家庭项目的 555 名成员制备的唾液样本中获得的 DNA 进行了比较。我们发现从全血中制备的 DNA 的性能明显优于从唾液中制备的 DNA。基因分型的成功与定量 PCR 测定的唾液样本中人类 DNA 的浓度显着相关，但与紫外光谱测定的 DNA 总量无关。我们的结论是，考虑选择用于基因分型研究的 DNA 来源材料的研究人员需要平衡基于唾液 DNA 采集方法的简便性和经济性，以及与从全血制备的 DNA 相关的更高的基因分型调用产量和速率。
Traditionally, large scale genotyping projects have used DNA derived from whole blood or lymphoblast cell lines. But over the past several years, a number of investigators have begun to use DNA prepared from saliva for genotyping studies, particularly for use in behavioral genetic studies. However, the comparability of DNA from these two sources has not been rigorously analyzed by unbiased sources. In this communication, we compare the single nucleotide polymorphism (SNP) genotyping results from DNA derived from whole blood samples obtained from 474 subjects from the Iowa Adoption Studies with that saliva samples prepared from 555 members of the Strong African American Families project. We found that DNA prepared from whole blood performed significantly better than that prepared from saliva. Genotyping success was significantly associated with the concentration of human DNA in the saliva sample as determined by quantitative PCR, but not with the total amount of DNA as determined by UV spectroscopy. We conclude that investigators contemplating the choice of source materials of DNA for genotyping studies will need to balance the ease and economy of saliva based DNA collection methods with the higher yields and rates of genotyping calls associated with DNA prepared from whole blood.