Decrease and gain of gene expression are equally discriminatory markers for prostate carcinoma -: A gene expression analysis on total and microdissected prostate tissue
Decrease and gain of gene expression are equally discriminatory markers for prostate carcinoma -: A gene expression analysis on total and microdissected prostate tissue
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DOI：
10.1016/s0002-9440(10)61165-0
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发表时间：
2002-06-01
影响因子：
6
通讯作者：
Gröne, HJ
中科院分区：
文献类型：
--
作者：
Ernst, T;Hergenhahn, M;Gröne, HJ
通过DNA微阵列分析来寻找前列腺癌相较于邻近正常组织中过表达和低表达基因的信息。对来自前列腺切除术获取的总共26个组织样本（17个未经治疗的前列腺癌样本，9个前列腺癌组织邻近的正常组织样本）的大约12600个mRNA序列进行了分析。进行了层次聚类分析。发现与邻近正常组织相比，在前列腺癌中有63个基因的表达水平显著（至少2.5倍）升高，而153个基因的表达降低（至少2.5倍）。除了先前描述的如hepsin等基因外，还发现了几个之前未受关注的基因过表达，例如编码特异性颗粒蛋白（SGP28）、α - 甲基 - 酰基辅酶A消旋酶、低密度脂蛋白（LDL） - 磷脂酶A2以及抗凋亡基因PYCR1的基因。放射敏感性基因ATDC以及编码DNA结合蛋白抑制剂ID1和磷脂酶抑制剂子宫珠蛋白的基因在癌症样本中显著下调。通过实时逆转录聚合酶链反应对8个基因的DNA微阵列数据在5个正常组织和5个癌症组织中进行了定量验证，两种方法之间具有高度相关性。对癌症和组织学正常标本的上皮和间质部分进行激光捕获显微切割，随后采用针对低水平RNA的扩增方案（
Information on over- and underexpressed genes in prostate cancer in comparison to adjacent normal tissue was sought by DNA microarray analysis. Approximately 12,600 mRNA sequences were analyzed from a total of 26 tissue samples (17 untreated prostate cancers, 9 normal adjacent to prostate cancer tissues) obtained by prostatectomy. Hierarchical clustering was performed. Expression levels of 63 genes were found significantly (at least 2.5-fold) increased, whereas expression of 153 genes was decreased (at least 2.5-fold) in prostate cancer versus adjacent normal tissue. in addition to previously described genes such as hepsin, overexpression of several genes was found that has not drawn attention before, such as the genes encoding the specific granule protein (SGP28), alpha-methyl-acyl-CoA racemase, low density lipoprotein (LDL)-phospholipase A2, and the anti-apoptotic gene PYCR1. The radiosensitivity gene ATDC and the genes encoding the DNA-binding protein inhibitor ID1 and the phospholipase inhibitor uteroglobin were significantly down-regulated in the cancer samples. DNA microarray data for eight genes were confirmed quantitatively in five normal and five cancer tissues by real-time reverse transcriptase-polymerase chain reaction with a high correlation between the two methods. Laser capture microdissection of epithelial and stromal. compartments from cancer and histological normal specimens followed by an amplification protocol for low levels of RNA (