Comparative investigation of sequentially extracted phosphorus fractions in a sandy loam soil and a swine manure
Comparative investigation of sequentially extracted phosphorus fractions in a sandy loam soil and a swine manure
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DOI：
10.1081/css-120021308
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发表时间：
2003-01-01
影响因子：
1.8
通讯作者：
Griffin, TS
中科院分区：
文献类型：
--
作者：
He, Z;Honeycutt, CW;Griffin, TS
连续分级法是研究土壤中磷形态的最常用方法之一。该策略可用于评价土壤磷的生物有效性，研究土壤磷转化与土壤发育的关系。近年来，人们采用顺序分级的方法研究了有机肥和堆肥中的磷及其在土壤中的应用变化。然而，动物粪便的物理化学性质可能与土壤不同。因此，需要进行评估，以确定根据土壤研究顺序提取的磷形态是否适用于粪便。在本研究中，我们用水、0.5M碳酸氢钠、0.1M氢氧化钠和1.0M盐酸对沙壤土和猪粪中的磷进行了分级。土壤中磷的分布为：水浸出物0.2%，碳酸氢钠浸出物11%，氢氧化钠浸提物58%，盐酸浸提物14%，残留磷16%。而猪粪中磷的分布为48%水浸提物，19%NaHCO3浸提物，18%NaOH浸提物，11%盐酸浸提物，3%残留磷。元素分析、紫外(UV)/可见光谱和傅里叶变换红外光谱(FT/IR)表明，猪粪中磷的化学组成与猪粪存在明显差异。土壤NaOH组分每千克干物质中铝(Al)和铁(Fe)的含量分别为128和5.8，而粪便的NaOH组分每千克干物质中铝和铁的含量仅为8.8和0.6。但不同形态的有机肥中钙(Ca)和镁(Mg)的含量均远高于土壤。土壤以无机矿物为主，动物粪便以有机残渣为主。这些数据表明，仅将NaOH可提取无机磷(P-I)分配给Al-P和Fe-P，将HCI-P-I分配给Ca-P，对猪粪进行土壤分级解释可能是不合适的。我们将土壤和粪便顺序分级所观察到的明显不同的磷分布模式归因于它们不同的物理化学性质。在制定和解释粪便分级程序时，必须认识到这些差异。
Sequential fractionation is one of the most common methods used to investigate phosphorus (P) forms in soils. The strategy can be used for evaluating bioavailability of soil P and for investigating the relationship between soil P transformation and soil development. Recently, the strategy of sequential fractionation has been used to investigate manure and compost P and their changes after application to soils. However, the physico-chemical characteristics of animal manure may differ from those of soils. Evaluation is therefore needed to determine if sequentially extracted P forms based on soil studies are applicable for manure. In this study we fractionated P in a sandy loam soil and a swine (Sus scrofa) manure with H2O, 0.5 M NaHCO3, 0.1 M NaOH, and 1.0 M HCl. The P distribution in soil was 0.2% H2O-extractable, 11% NaHCO3-extractable, 58% NaOH-extractable, 14% HCl-extractable, and 16% residual P. In contrast, P distribution in swine manure was 48% H2O-extractable, 19% NaHCO3-extractable, 18% NaOH-extractable, 11% HCl-extractable, and 3% residual P. Elemental analyses, ultraviolet (UV)/visible spectra, and Fourier-transform infrared (FT/IR) spectra revealed distinct differences in chemical composition between soil and swine manure. The NaOH fraction of soil contained 128 mmol aluminum (Al) and 5.8 mmol iron (Fe) per kg of dry matter; however, the NaOH fraction of manure contained only 8.8 mmol Al and 0.6 mmol Fe per kg of dry matter. Concentrations of calcium (Ca) and magnesium (Mg) in various fractions of manure, however, were much higher than in soil. The soil was inorganic mineral-based, and the animal manure was organic residue-based. These data indicate it may not be appropriate to apply soil based fractionation interpretations to swine manure by exclusively assigning NaOH-extractable inorganic P (P-i) to Al- and Fe-P, and HCI-extractable-P-i to Ca-P. We attribute the distinctly different P distribution patterns observed with sequential fractionation of soil and manure to their different physico-chemical properties. These differences must be recognized when developing and interpreting fractionation procedures for manure.