The effect of aging on pars compacta of the substantia nigra in rhesus monkey.
The effect of aging on pars compacta of the substantia nigra in rhesus monkey.
复制标题
衰老对恒河猴黑质致密部的影响。
DOI：
10.1097/00005072-199909000-00002
复制
发表时间：
1999
影响因子：
3.2
通讯作者：
Peters,A
中科院分区：
文献类型：
--
作者：
Siddiqi,ZA;Peters,A
本研究旨在重新评估恒河猴黑质致密部神经元的类型，进而确定衰老对神经元和神经胶质细胞形态的影响。因此，在高尔基浸渍材料中，在标记有酪氨酸羟化酶和GABA抗体的切片中，以及在塑料包埋的组织中进行光镜和电子显微镜检查黑质。神经元有三种类型：(1)大型多极神经元具有突出的尼索尔体；(2)双极神经元中等大小，呈梭形，尼索尔体局限于深凹的核包膜的两极；(3)小型多极细胞具有稀疏的尼索尔物质。大、中神经元为多巴胺能神经元，小神经元为氨基丁酸能神经元。随着年龄的增长，所有的神经元都在积累脂褐素，尤其是小的多极神经元。此外，Marinesco小体出现在大型多极神经元的核内。树突受年龄的影响最为严重；许多细胞失去细胞器，细胞质充满液泡、膜状螺旋和致密体。在年老的猴子中，许多星形胶质细胞有包涵体，在神经细胞中，星形胶质细胞突起的数量显著增加。大的球体也出现了。这些细胞似乎来自星形胶质细胞，它们有一个致密颗粒状物质的核心，周围是细胞质的浅色外围带，突起可以从那里延伸。大多数少突胶质细胞的细胞质中有致密的包涵体，许多髓鞘被破坏。小胶质细胞可因吞噬物质而严重肿胀。尽管神经元和神经胶质细胞都受到年龄的影响，但没有发现可以被解释为死亡神经元的实体。
This aim of this study was to re-evaluate the types of neurons present in pars compacta of the substantia nigra in the rhesus monkey, and then to determine the effects of aging on the morphology of both the neurons and neuroglial cells. The substantia nigra was therefore examined in Golgi impregnated material, in sections labeled with antibodies to tyrosine hydroxylase and to GABA, and in tissue embedded in plastic for light and electron microscopy. Three types of neurons were encountered: (1) large multipolar neurons with prominent Nissl bodies, (2) bipolar neurons that are medium sized and spindle-shaped, with Nissl bodies confined to the poles of the deeply indented nuclear envelopes, and (3) small multipolar cells with sparse Nissl substance. The large and medium neurons are believed to be dopaminergic and the small neurons GABA-ergic. With age, all of the neurons accumulate lipofuscin, especially the small multipolar neurons. In addition, Marinesco bodies appear within the nuclei of the large multipolar neurons. The dendrites are most severely affected by age; many of them lose organelles and their cytoplasm can become filled with vacuoles, membranous whorls, and dense bodies.In old monkeys many of the astrocytes have inclusions and in the neuropil there is a striking increase in the number of astrocytic processes. Also large spheroids appear. These appear to be derived from astrocytes and they have a core of dense granular material surrounded by a paler peripheral zone of cytoplasm from which processes can extend. Most of the oligodendrocytes have dense inclusions in their cytoplasm and many of the myelin sheaths break down. Microglial cells can become enormously swollen by phagocytosed material. Although both neurons and neuroglial cells are affected by age, no entities that could be construed to be dying neurons were encountered.