Snail is an independent prognostic predictor for progression and patient survival of gastric cancer
Snail is an independent prognostic predictor for progression and patient survival of gastric cancer
复制标题
Snail 是胃癌进展和患者生存的独立预后预测因子
DOI：
10.1111/j.1349-7006.2012.02295.x
复制
发表时间：
2012-07-01
期刊：
影响因子：
5.7
通讯作者：
Sun, Yihong
中科院分区：
文献类型：
--
作者：
He, Hongyong;Chen, Weidong;Sun, Yihong
本研究探讨了锌指转录因子Snail在胃癌发生和发展中的临床意义。为了阐明胃癌细胞中Snail表达和去分化状态与肿瘤干细胞表型之间的关系，我们采用了蛋白质印迹分析、逆转录 - 聚合酶链反应、实时定量聚合酶链反应和流式细胞术。对10例人类正常胃组织样本和103例具有临床病理特征的胃癌组织进行免疫组织化学染色及Snail表达评估，随后进行统计分析，以评估Snail表达对胃癌进展和患者生存的预后价值。结果表明，功能性Snail表达将胃癌细胞的去分化状态与肿瘤干细胞表型相互联系起来。此外，胃癌组织中Snail的表达水平与肿瘤细胞分化、局部肿瘤生长、淋巴结状态、远处转移和肿瘤分期显著相关。Snail高表达的胃癌患者的总体生存率显著低于Snail低表达的患者。多变量Cox回归分析表明，Snail表达是胃癌患者生存的独立预后预测因子。因此，我们的数据表明，Snail表达可能是预测胃癌进展的一个可靠的独立预后因素，这可能为对胃癌患者的功能性Snail表达进行潜在的临床干预开辟一条新途径。（《癌症科学》2012年；103卷：1296 - 1303页）
The present study investigated the clinical significance of Snail, a zinc-finger transcription factor, in the development and progression of gastric cancer. To elucidate the relationship between Snail expression and dedifferentiation status with cancer stem cell phenotype in gastric cancer cells, we used western blot analysis, RT-PCR, quantitative real-time PCR and flow cytometry. Immunohistochemistry staining and evaluation of Snail expression in 10 human normal gastric samples versus 103 clinicopathologically characterized gastric cancer tissues followed by statistical analyses were applied to evaluate the prognostic value of Snail expression for progression and patient survival of gastric carcinomas. The results showed that functional Snail expression interlinks dedifferentiation status with cancer stem cell phenotype in gastric cancer cells. In addition, expression levels of Snail in gastric cancer tissues were significantly associated with tumor cell differentiation, local tumor growth, lymph node status, distant metastasis and tumor stage. The overall survival rate of gastric cancer patients with high Snail expression was significantly lower than for those patients with low Snail expression. Multivariate Cox regression analyses showed that Snail expression is an independent prognostic predictor for patient survival of gastric carcinomas. Thus, our data suggest that Snail expression could be a reliable independent prognostic factor to predict gastric carcinoma progression, which might open a new avenue for potential clinical intervention with functional Snail expression in gastric cancer patients. (Cancer Sci 2012; 103: 12961303)