Preparation and characterization of WO3/TiO2 hollow microsphere composites with catalytic activity in dark
Preparation and characterization of WO3/TiO2 hollow microsphere composites with catalytic activity in dark
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DOI：
10.1016/j.cej.2010.12.007
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发表时间：
2012-02
影响因子：
15.1
通讯作者：
Yanting Li;Lei Chen;Ya-nan Guo;Xiuguo Sun;Yu Wei
中科院分区：
文献类型：
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作者：
Yanting Li;Lei Chen;Ya-nan Guo;Xiuguo Sun;Yu Wei
采用水包油包水W/O/W乳化溶剂萃取法，将从金环蛇（Bungarus fasciatus）蛇毒中分离得到的含有30个氨基酸残基的水溶性多肽Cathelicidin-BF-30（BF-30）包埋在聚（D，L-丙交酯-乙交酯）（PLGA）75：25微球（MS）中。本工作的目的是研究包封后BF-30的稳定性。D-海藻糖用作赋形剂以稳定肽。所得MS粒径大多在2 μ m以下，包封率为88.5061。百分之二十九使用圆二色性（CD）确定体外释放的肽的二级结构与天然肽几乎相同。BF-30抑制大肠杆菌生长的能力也得以保持。细胞相对生长率和溶血率分别为92.1663。55%和3.5260。分别为45%。
Cathelicidin-BF-30 (BF-30), a water-soluble peptide isolated from the snake venom of Bungarus fasciatus containing 30 amino acid residues, was incorporated in poly (D, L-lactide-co-glycolide)(PLGA) 75: 25 microspheres (MS) prepared by a water in oil in water W/O/W emulsification solvent extraction method. The aim of this work was to investigate the stability of BF-30 after encapsulation. D-trehalose was used as an excipient to stabilize the peptide. The MS obtained were mostly under 2 цm in size and the encapsulation efficiency was 88.5061. 29%. The secondary structure of the peptide released in vitro was determined to be nearly the same as the native peptide using Circular Dichroism (CD). The ability of BF-30 to inhibit the growth of Escherichia coli was also maintained. The cellular relative growth and hemolysis rates were 92.1663. 55% and 3.5260. 45% respectively.