Shifting the IGF-axis: An age-related decline in human tear IGF-1 correlates with clinical signs of dry eye.
Shifting the IGF-axis: An age-related decline in human tear IGF-1 correlates with clinical signs of dry eye.
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DOI：
10.1016/j.ghir.2018.02.001
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Robertson DM
中科院分区：
文献类型：
--
作者：
Patel R;Zhu M;Robertson DM
人角膜上皮既表达胰岛素样生长因子1型受体（IGF-1R），也表达IGF-1R/胰岛素受体（INSR）杂交体。尽管之前在人泪液中发现了IGF-1，但关于泪液中IGF-1的调节及其在角膜上皮稳态中的作用知之甚少。在本研究中，我们研究了生物参数对人泪液中IGF-1浓度水平的影响。在41名没有任何干眼症状的健康志愿者中测量了IGF-1的泪液水平。所有志愿者都接受了角膜和泪膜的标准生物显微镜检查。在志愿者的一个亚组中，用荧光素钠对角膜进行染色、泪膜破裂时间和泪液生成进行测量，以评估干眼症的临床症状。采用玻璃微毛细管采集下半月板泪液，采用固相夹心ELISA法检测IGF-1水平。IGF-1的撕裂水平在年轻人中最高，在老年人中显著降低（P=0.003）。泪液IGF-1水平在男性和女性之间无显著差异（P=0.628）。泪液igf水平与泪膜破裂时间（R=0.738）和泪液产生量（R=0.826）相关。这些数据表明，由于衰老，泪液中IGF-1的逐渐下降与干眼症的临床症状有关。这种影响可能是由于与年龄相关的泪腺变化。
The human corneal epithelium expresses both the insulin-like growth factor type 1 receptor (IGF-1R) and the IGF-1R/insulin receptor (INSR) hybrid. Despite the previous identification of IGF-1 in human tear fluid, little is known regarding the regulation of IGF-1 in tear fluid and its role in corneal epithelial homeostasis. In the present study, we investigated the impact of biological parameters on the concentration of human tear levels of IGF-1. Tear levels of IGF-1 were measured in 41 healthy, human volunteers without any reported symptoms of dry eye. All volunteers underwent standard biomicroscopic examination of the cornea and tear film. In a subgroup of volunteers, corneal staining with sodium fluorescein, tear film break up time and tear production using a Schirmer’s test strip were measured to assess clinical signs of dry eye. Tears were collected from the inferior tear meniscus using glass microcapillary tubes and IGF-1 levels were measured using a solid phase sandwich ELISA. Tear levels of IGF-1 were highest in young adults and significantly decreased in older adults (P=0.003). There were no differences in tear IGF-1 between males and females (P=0.628). Tear IGF-levels were correlated with tear film break up time (R=0.738) and tear production (R=0.826). These data indicate that there is a progressive decline in tear IGF-1 due to aging that is associated with clinical signs of dry eye. This effect is likely due to age-related changes in the lacrimal gland.
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DOI：
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发表时间：
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影响因子：
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发表时间：
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