Exploring the relationship between the signal molecule AI-2 and the biofilm formation of Lactobacillus sanfranciscensis
Exploring the relationship between the signal molecule AI-2 and the biofilm formation of Lactobacillus sanfranciscensis
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DOI：
10.1016/j.lwt.2021.112704
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发表时间：
2021-10
期刊：
影响因子：
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通讯作者：
Yue Zhang;Yue Gu;Rong Wu;Yanxue Zheng;Yan Wang;Lili Nie;R. Qiao;Yinfeng He
中科院分区：
文献类型：
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作者：
Yue Zhang;Yue Gu;Rong Wu;Yanxue Zheng;Yan Wang;Lili Nie;R. Qiao;Yinfeng He
本研究的目的是研究信号分子AI-2对桑氏乳杆菌生物膜形成的影响。我们发现，信号分子AI-2对菌株的影响与多种生理代谢行为有关，如细菌生长、形态特征、生物膜发育、相关基因表达以及胆盐胁迫耐受性等。浮游生物膜和生物膜的存活率。在酸、胆盐和体外消化后测定肝素敏感性。结果表明，生物膜菌株具有较强的抗应力能力。其次，内源性和人工合成的AI-2 ONL的作用。比较了三种生长方式对生长的影响。人工合成AI-2的加入提高了桑氏杆菌的细胞密度，增强了细菌的凝聚力。研究发现，HsH3基因的表达上调可促进生物被膜的形成和生长。AI-2干预可提高INL对胆盐的耐受性。桑弗兰西斯科。综上所述，信号分子AI-2参与了INL生物膜形成和发育的调控。桑弗兰西斯科。
The objective of this study was to investigate the effect of the signal molecule AI-2 on biofilm formation inLactobacillus sanfranciscensis. We found that the influence of signal molecule AI-2 on strains is related to a variety of physiological metabolic behaviors, such as bacterial growth, morphological characteristics, biofilm development, related gene expression, and bile salt stress tolerance. The survival rates of planktonic and biofilm forms ofL. sanfranciscensiswere measured after acid, bile salt, andin vitrodigestion. The results showed that the biofilm strain had stronger stress resistance. Next, the effects of endogenous and synthetic AI-2 onL. sanfranciscensisgrowth were compared. The addition of synthetic AI-2 improvedL.sanfranciscensiscell density and enhanced bacterial cohesion. Upregulated expression offtsHwas found to promote biofilm formation and growth. AI-2 intervention will improve bile salt tolerance inL. sanfranciscensis. In conclusion, the signal molecule AI-2 is involved in the regulation of biofilm formation and development inL. sanfranciscensis.