Class I H-2Db determinants are not involved in hybrid resistance to parental H-2b/Hh-1b bone marrow allograft.
Class I H-2Db determinants are not involved in hybrid resistance to parental H-2b/Hh-1b bone marrow allograft.
复制标题
I 类 H-2Db 决定簇不参与对亲本 H-2b/Hh-1b 骨髓同种异体移植物的杂种抗性。
DOI：
10.1002/eji.1830170722
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发表时间：
1987
影响因子：
5.4
通讯作者：
Nakamura,I
中科院分区：
文献类型：
--
作者：
Milisauskas,VK;Kaminsky,SG;Nakamura,I
对亲本 H-2bb 骨髓移植物的杂交抗性针对由 H-2D 区域内或附近的 Hh-1 基因座控制的细胞表面结构。这种表面结构的性质尚不清楚。由于该单倍型中的 I 类 H-2D 基因座或基因座的纯合性似乎是移植物对抗性敏感的必要条件，但不是充分条件，因此我们测试了该表型的表达是否依赖于 I 类 H-2Db 决定簇的表达。 H-2b 肿瘤 RBL-5 的克隆变体是通过免疫选择获得的，因为不存在 H-2Db 表达，这是通过无法结合特异性抗体以及诱导或与同种异体反应性细胞毒性 T 淋巴细胞反应来确定的。本研究中使用的三个克隆是 H-2Db 阴性但 H-2Kb 阳性，并且具有自然杀伤细胞抗性。当作为竞争性抑制剂进行体内测试时，变异细胞能够阻断对亲本 H-2bb 骨髓移植物的杂种抗性，就像未选择的 H-2Db 阳性亲本系细胞一样。因此，H-2Db 表达与 Hh-1b 表达无关。一个偶然的观察结果是，YAC-1 细胞（一种对自然杀伤明显敏感的非 H-2b 肿瘤）能够阻断杂交耐药性。这种反应性在我们之前的研究中没有观察到，这提出了至少一些效应细胞具有交叉反应性或能够双重识别的可能性。
Hybrid resistance to parental H‐2bbone marrow grafts is directed to a cell surface structure controlled by the Hh‐1 locus in or near the H‐2D region. The nature of this surface structure is not known. Since homozygosity at the class I H‐2D locus or loci in this haplotype would seem a necessary but not sufficient condition for the grafts' susceptibility to resistance, we tested whether the expression of this phenotype is dependent on the expression of class I H‐2Dbdeterminants. Cloned variants of H‐2btumor RBL‐5 were obtained by immunoselection for the absence of H‐2Dbexpression, as determined by the inability to bind specific antibody and to induce or react with alloreactive cytotoxic T lymphocytes. The three clones used in this study were H‐2Dbnegative but H‐2Kbpositive and were natural killer cell resistant. When testedin vivoas competitive inhibitors the variant cells were capable of blocking hybrid resistance to parental H‐2bbone marrow grafts as were unselected H‐2Db‐positive parental line cells. Therefore, H‐2Dbexpression is irrelevant for Hh‐1bexpression. An incidental observation was that YAC‐1 cells, a non‐H‐2btumor with pronounced susceptibility to natural killing, were able to block hybrid resistance. This reactivity, not observed in our previous studies, raises the possibility that at least some of the effector cells are cross‐reactive or capable of dual recognition.