NEW LEAD CITRATE METHOD FOR ULTRACYTOCHEMICAL DEMONSTRATION OF ACTIVITY OF NON-SPECIFIC ALKALINE PHOSPHATASE (ORTHOPHOSPHORIC MONOESTER PHOSPHOHYDROLASE)

NEW LEAD CITRATE METHOD FOR ULTRACYTOCHEMICAL DEMONSTRATION OF ACTIVITY OF NON-SPECIFIC ALKALINE PHOSPHATASE (ORTHOPHOSPHORIC MONOESTER PHOSPHOHYDROLASE)
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DOI:
10.1007/bf00326615
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发表时间:
1967-01-01
期刊:
HISTOCHEMIE
影响因子:
--
通讯作者:
OGAWA, K
OGAWA, K
中科院分区:
其他
文献类型:
--
作者:
MAYAHARA, H;HIRANO, H;OGAWA, K

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介绍了利用“柠檬酸铅”作为捕获试剂,在高碱性pH范围内进行非特异性碱性磷酸酶活性超细胞化学验证的新方法。本文介绍了用新的“柠檬酸铅”法在大鼠各组织中得到的结果,并结合其他方法的观察结果进行了讨论。该方法也可应用于光显微水平。
The new method, utilizing “lead citrate” as capture reagent, for the ultracytochemical demonstration of non-specific alkaline phosphatase activity at high alkaline ranges of pH was introduced. Results obtained by the new “lead citrate” method in various tissues of the rat were presented and discussed in connection with observations made by other methods. The method can be used at the light microscopic level also.