Oxygen-Releasing Microparticles for Cell Survival and Differentiation Ability under Hypoxia for Effective Bone Regeneration
Oxygen-Releasing Microparticles for Cell Survival and Differentiation Ability under Hypoxia for Effective Bone Regeneration
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DOI：
10.1021/acs.biomac.8b01760
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发表时间：
2019-02-01
影响因子：
6.2
通讯作者：
Oh, Se Heang
中科院分区：
文献类型：
--
作者：
Kim, Ho Yong;Kim, So Young;Oh, Se Heang
将足够的氧气输送到组织工程化三维（3D）支架中以产生临床适用的组织/器官仍然是研究人员和临床医生的挑战。克服这种限制的一种潜在策略是使用氧释放支架。在本研究中，我们制备了中空微粒（HPs）与氧载体全氟辛烷（PFO; PFO-HPs）的乳液加载的及时供应氧气到周围的细胞。这些PFO-HPs延长了缺氧条件下人骨膜衍生细胞（hPDCs）的存活时间并保持了其成骨分化能力。接种到PFO-HP上的hPDC以更快的速度形成新骨，并且比接种到磷酸盐缓冲盐水加载的对照HP上的hPDC具有更高的骨密度。这些发现表明，PFO-HPs为hPDCs在无血管网络的骨缺损处的存活和维持分化能力提供了合适的环境，直到新血管长入发生，从而增强骨再生。PFO-HP是一种有前途的系统，用于有效递送各种功能细胞，包括干细胞和祖细胞，以再生受损的组织/器官。
Sufficient oxygen delivery into tissue-engineered three-dimensional (3D) scaffolds to produce clinically applicable tissues/organs remains a challenge for researchers and clinicians. One potential strategy to overcome this limitation is the use of an oxygen releasing scaffold. In the present study, we prepared hollow microparticles (HPs) loaded with an emulsion of the oxygen carrier perfluorooctane (PFO; PFO-HPs) for the timely supply of oxygen to surrounding cells. These PFO-HPs prolonged the survival and preserved the osteogenic differentiation potency of human periosteal-derived cells (hPDCs) under hypoxia. hPDCs seeded onto PFO-HPs formed new bone at a faster rate and with a higher bone density than hPDCs seeded onto phosphate buffered saline-loaded control HPs. These findings suggest that PFO-HPs provide a suitable environment for the survival and maintenance of differentiation ability of hPDCs at bony defects without vascular networks until new blood vessel ingrowth occurs, thus enhancing bone regeneration. PFO-HPs are a promising system for effective delivery of various functional cells, including stem cells and progenitor cells, to regenerate damaged tissues/organs.