Chromatin architecture underpinning transcription elongation
Chromatin architecture underpinning transcription elongation
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DOI：
10.1080/19491034.2016.1200770
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发表时间：
2016-01-01
期刊：
影响因子：
3.7
通讯作者：
Blobel, Gerd A.
中科院分区：
文献类型：
--
作者：
Lee, Kiwon;Blobel, Gerd A.
RNA聚合酶2（pol2）与增强子和启动子结合，随后是转录起始和随后的暂停。在释放时，pol2进行生产性伸长。广泛传播的转录观点认为，在延伸过程中，pol2和相关因子清除启动子近端区域，沿着染色质纤维沿着追踪，直到遇到终止位点。然而，一些研究与替代模型兼容。这些模型中的一个共同特征是转录延伸是基因沿着由pol2和相关因子组成的复合物移动的结果。这种情况预测，活性增强子和启动子，转录复合物，包括pol2的结合是在动态物理接近的基因体的方式平行pol2的持续合成能力。在同步转录条件下，通过染色体构象捕获确实观察到了这一点。在这里，我们将讨论这些意见和他们的建筑模型的转录延伸的影响。
RNA polymerase 2 (pol2) associates with enhancers and promoters, followed by transcription initiation and subsequent pausing. Upon release, pol2 proceeds into productive elongation. A wide spread view of transcription holds that during elongation, pol2 and associated factors clear the promoter proximal region to track along the chromatin fiber until a termination site is encountered. However, several studies are compatible with alternative models. One common feature among these models is that transcription elongation results from movement of the gene along a complex consisting of pol2 and associated factors. Such a scenario predicts that active enhancers and promoters that are bound by transcription complexes, including pol2 are in dynamic physical proximity with the gene body in a manner paralleling pol2 processivity. This has indeed been observed by chromosome conformation capture under conditions of synchronous transcription. Here we discuss these observations and their implication for architectural models of transcription elongation.