3 RAT PREPROTACHYKININ MESSENGER-RNAS ENCODE THE NEUROPEPTIDES SUBSTANCE-P AND NEUROKININ-A
3 RAT PREPROTACHYKININ MESSENGER-RNAS ENCODE THE NEUROPEPTIDES SUBSTANCE-P AND NEUROKININ-A
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DOI：
10.1073/pnas.84.3.881
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发表时间：
1987-02-01
影响因子：
11.1
通讯作者：
HERSHEY, AD
中科院分区：
文献类型：
--
作者：
KRAUSE, JE;CHIRGWIN, JM;HERSHEY, AD
使用合成寡核苷酸筛选大鼠纹状体 cDNA 文库中对应于速激肽 P 物质和神经激肽 A 的序列。该 cDNA 文库是由从大鼠纹状体的头侧部分（纹状体黑质细胞体的部位）分离的 RNA 构建的。通过限制酶分析和 DNA 测序分离和定义了两种类型的 cDNA，以编码 P 物质和神经激肽 A。两种预测的前原速激肽蛋白前体（长度为 130 和 115 个氨基酸）因两个速激肽序列之间的十五肽序列而彼此不同，并且两种前体都具有 P 物质和神经激肽 A 的适当处理信号 生产。通过 S1 核酸酶保护实验确定了大鼠纹状体中存在少量丰度的第三种前原速激肽 mRNA。该 mRNA 编码一种由 112 个氨基酸组成的前速激肽，包含 P 物质，但不包含神经激肽 A。这三种 mRNA 源自一个大鼠基因，是差异 RNA 加工的结果；因此，这种RNA加工模式进一步增加了前原速激肽基因产生的产物的多样性。
Synthetic oligonucleotides were used to screen a rat striatal cDNA library for sequences corresponding to the tachykinin peptides substance P and neurokinin A. The cDNA library was constructed from RNA isolated from the rostral portion of the rat corpus striatum, the site of striatonigral cell bodies. Two types of cDNAs were isolated and defined by restriction enzyme analysis and DNA sequencing to encode both substance P and neurokinin A. The two predicted preprotachykinin protein precursors (130 and 115 amino acids in length) differ from each other by a pentadecapeptide sequence between the two tachykinin sequences, and both precursors possess appropriate processing signals for substance P and neurokinin A production. The presence of a third preprotachykinin mRNA of minor abundance in rat striatum was established by S1 nuclease protection experiments. This mRNA encodes a preprotachykinin of 112 amino acids containing substance P but not neurokinin A. These three mRNAs are derived from one rat gene as a result of differential RNA processing; thus, this RNA processing pattern further increases the diversity of products that can be generated from the preprotachykinin gene.