Heat shock protein 70 is involved in malignant behaviors and chemosensitivities to cisplatin in cervical squamous cell carcinoma cells.
Heat shock protein 70 is involved in malignant behaviors and chemosensitivities to cisplatin in cervical squamous cell carcinoma cells.
复制标题
热休克蛋白 70 参与宫颈鳞状细胞癌细胞的恶性行为和对顺铂的化学敏感性。
DOI：
10.1111/jog.12325
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Sugino N
中科院分区：
文献类型：
--
作者：
Yoshidomi K;Murakami A;Yakabe K;Sueoka K;Nawata S;Sugino N
目的 先前通过蛋白质组学分析发现，与正常组织相比，热休克蛋白 70 (HSP70) 在宫颈鳞状细胞癌 (SCC) 组织中高表达。本研究探讨了HSP70在体外宫颈鳞状细胞癌细胞恶性行为和顺铂化疗敏感性中的作用。方法使用siRNA在两种人宫颈癌细胞（SiHa和SKG II）中检测HSP70敲低对细胞迁移、细胞侵袭和细胞增殖、细胞周期状态和凋亡活性的影响。此外，还检测了HSP70敲低对顺铂凋亡效应的影响。结果HSP70敲低显着抑制细胞迁移、细胞侵袭和细胞增殖活性。细胞周期被阻滞在 S 期和 G2/M 期，HSP70 敲低导致细胞凋亡增加。尽管顺铂在对照细胞中没有凋亡作用，但在 SiHa 的 HSP70 敲低细胞中显示出剂量依赖性凋亡作用。在SKG II中，5μg/mL的顺铂诱导细胞凋亡，并且HSP70敲低增强了顺铂的凋亡作用。结论HSP70在宫颈SCC的进展中发挥了功能性作用，HSP70敲低增强了宫颈SCC细胞对顺铂的化疗敏感性。
AimHeat shock protein 70 (HSP70) was previously found to be highly expressed in cervical squamous cell carcinoma (SCC) tissues compared with normal tissues by proteomic analyses. The present study investigated the roles of HSP70 in malignant behaviors and chemosensitivity to cisplatin in cervical SCC cellsin vitro.MethodsEffects of HSP70 knockdown on activities of cell migration, cell invasion and cell proliferation, cell cycle status, and apoptosis were examined using siRNA in two human cervical cancer cells (SiHa and SKG II). Furthermore, the effect of HSP70 knockdown on the apoptotic effect by cisplatin was examined.ResultsHSP70 knockdown significantly suppressed activities of cell migration, cell invasion and cell proliferation. The cell cycle was arrested at the S‐ and G2/M‐phases with the increase in apoptosis by HSP70 knockdown. Although cisplatin had no apoptotic effect in the control cell, it showed a dose‐dependent apoptotic effect in the HSP70 knockdown cells in SiHa. In SKG II, 5 μg/mL of cisplatin induced apoptosis, and the apoptotic effect by cisplatin was enhanced by HSP70 knockdown.ConclusionIt was suggested that HSP70 played functional roles in the progression of uterine cervical SCC, and HSP70 knockdown enhanced chemosensitivities to cisplatin in cervical SCC cells.