Evaluation of blood compatibility of plasma deposited heparin-like films and SF6 plasma treated surfaces
Evaluation of blood compatibility of plasma deposited heparin-like films and SF6 plasma treated surfaces
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DOI：
10.1590/s1516-14392010000100019
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发表时间：
2010
影响因子：
1.7
通讯作者：
I. A. Perrenoud;E. Rangel;R. Mota;Steven Frederick Durrant;N. Cruz
中科院分区：
文献类型：
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作者：
I. A. Perrenoud;E. Rangel;R. Mota;Steven Frederick Durrant;N. Cruz
在用于心脏直视手术和透析的设备中，血液必须使用由蠕动泵和活性组件（如特定过滤器和氧合器）组成的体外回路连续处理。为了避免与这种装置的人造表面接触而引起的血液凝固，已经采用了几种方法。通常使用肝素，一种能够防止血栓形成的生物活性蛋白。在这项工作中，我们使用含肝素的气体等离子体来评估在PVC和玻璃表面沉积粘附抗凝膜的可能性。采用射频等离子体增强化学气相沉积技术将肝素/异丙醇和肝素/六甲基二硅氧烷溶液制备成薄膜。此外，还研究了SF6等离子体暴露对这些表面相容性的影响。通过测定新鲜采集的人血和与不同表面接触2.5小时后的血小板密度、纤维蛋白原密度、活化的部分凝血活素（APTT）和凝血酶原时间（PT）来评价血液相容性。并用红外光谱、接触角和表面能对沉积膜进行了表征。与未处理的样品相比，将血液放置在涂有肝素/异丙醇混合物PECVD膜的玻璃基板上，以及与SF6血浆处理过的PVC接触，血液的凝固时间分别增加了约60%和20%。
In devices used in open-heart surgery and dialysis, blood must be continuously processed using extracorporeal circuits composed of peristaltic pumps and active components such as specific filters and oxygenators. Several procedures have been employed to avoid blood coagulation induced by contact with the artificial surfaces of such devices. Often heparin, a bioactive protein able to prevent clot formation, is employed. In this work, we have used heparin-containing gas plasmas to evaluate the possibility of depositing adherent anticoagulant films onto PVC and glass surfaces. The films were produced by radiofrequency plasma enhanced chemical vapor deposition from heparin/isopropanol and heparin/hexamethyldisiloxane solutions. In addition, the effects of exposure to SF6 plasmas on the compatibility of such surfaces have also been investigated. The blood compatibility was evaluated through the determination of the density of platelets and fibrinogen and activated partial thromboplastin (APTT) and prothrombin times (PT) of human blood freshly collected and after contact for 2.5 hours with different surfaces. The deposited films were also characterized by infrared spectroscopy, contact angle and surface energy measurements. The coagulation time of blood, placed in contact with glass substrates coated by PECVD films of heparin/isopropanol mixtures, and in contact with SF6 plasma-treated PVC, increased by about 60 and 20%, respectively, compared to the values measured with untreated samples.