INDUCTION BY EGF AND INTERFERON-GAMMA OF TYROSINE-PHOSPHORYLATED DNA-BINDING PROTEINS IN MOUSE-LIVER NUCLEI
INDUCTION BY EGF AND INTERFERON-GAMMA OF TYROSINE-PHOSPHORYLATED DNA-BINDING PROTEINS IN MOUSE-LIVER NUCLEI
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DOI：
10.1126/science.8378774
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发表时间：
1993-09-24
期刊：
影响因子：
56.9
通讯作者：
COHEN, S
中科院分区：
文献类型：
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作者：
RUFFJAMISON, S;CHEN, K;COHEN, S
腹膜内注射表皮生长因子（EGF）到小鼠导致出现在肝细胞核中的三个酪氨酸磷酸化蛋白（84，91，和92千道尔顿）的EGF给药后几分钟内。给药干扰素-γ（IFN-γ）导致肝细胞核中出现两种酪氨酸磷酸化蛋白（84和91千道尔顿）。在EGF或IFN-γ给药后检测到的84和91千道尔顿蛋白被鉴定为IFN-γ活化因子（GAF）。此外，凝胶位移分析显示，这些GAF蛋白，检测后，无论是EGF或IFN-γ管理，特异性结合到c-fos启动子的SIS诱导元件。因此，GAF蛋白参与IFN-γ和EGF途径中的核信号传导。
Intraperitoneal injection of epidermal growth factor (EGF) into mice resulted in the appearance in liver nuclei of three tyrosine phosphorylated proteins (84, 91, and 92 kilodaltons) within minutes after administration of EGF. Administration of interferon-gamma (IFN-gamma) resulted in the appearance in liver nuclei of two tyrosine phosphorylated proteins (84 and 91 kilodaltons). The 84- and 91-kilodalton proteins detected after either EGF or IFN-gamma administration were identified as the IFN-gamma activation factors (GAF). Furthermore, gel shift analysis revealed that these GAF proteins, detected after either EGF or IFN-gamma administration, specifically bound to the sis-inducible element of the c-fos promoter. Thus, GAF proteins participate in nuclear signaling in both IFN-gamma and EGF pathways.