Leukocyte deformability is a novel biomarker to reflect sepsis-induced disseminated intravascular coagulation
Leukocyte deformability is a novel biomarker to reflect sepsis-induced disseminated intravascular coagulation
复制标题
白细胞变形能力是反映脓毒症引起的弥散性血管内凝血的新型生物标志物
DOI：
10.1002/ams2.54
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发表时间：
2015
影响因子：
1.6
通讯作者：
Tanaka H.
中科院分区：
文献类型：
--
作者：
Morikawa M;Inoue Y;Sumi Y;Kuroda Y;Tanaka H.
目的脓毒症引起的弥散性血管内凝血与高死亡率相关。脓毒症患者多形核白细胞功能和变形能力的变化。本研究的目的是描述脓毒症引起的弥散性血管内凝血病患者多形核白细胞变形能力的变化，并评估弥散性血管内凝血病的严重程度与多形核白细胞变形能力之间的关系。这些患者被诊断为重度脓毒症和急性弥散性血管内凝血病评分≥ 4。在第1、3和7天从这些患者中采集血液样品。用微通道流动分析仪测量多形性白细胞变形性，用流式细胞术测量多形性白细胞活性，以CD11b表示。与此相反，14例符合脓毒症标准，但没有复杂的弥散性血管内凝血病的患者也进入了这项研究。ResultsIn脓毒症引起的弥散性血管内凝血病的患者，有一个显着的相关性，他们的日本急性医学协会弥散性血管内凝血评分和多形核白细胞变形能力，和CD11b表达。多形核白细胞变得更加僵硬和CD11b的表达是更高的脓毒症引起的弥漫性血管内凝血病的患者相比，没有condition.Conclusionpolymorphism白细胞变形性与脓毒症引起的弥漫性血管内凝血病的严重程度和对治疗的反应。
AimSepsis‐induced disseminated intravascular coagulopathy is associated with a high mortality rate. The function and deformability of polymorphonuclear leukocytes change in patients with sepsis. The goal of this study was to characterize the changes in polymorphonuclear leukocyte deformability in patients with sepsis‐induced disseminated intravascular coagulopathy and to evaluate the relationship between the severity of disseminated intravascular coagulopathy and the deformability of polymorphonuclear leukocytes.MethodsThirty‐five patients with sepsis‐induced disseminated intravascular coagulopathy at our department were enrolled in this study. These patients were diagnosed with severe sepsis and an acute disseminated intravascular coagulopathy score ≥ 4. Blood samples were obtained from these patients on days 1, 3, and 7. Polymorphonuclear leukocyte deformability was measured with a microchannel flow analyzer, and polymorphonuclear leukocyte activity, represented as CD11b, was measured by flow cytometry. In contrast, 14 patients who fulfilled with sepsis criteria but without complicated disseminated intravascular coagulopathy were also entered in this study.ResultsIn patients with sepsis‐induced disseminated intravascular coagulopathy, there was a significant correlation between their Japanese Association for Acute Medicine disseminated intravascular coagulopathy score and polymorphonuclear leukocyte deformability, and CD11b expression. Polymorphonuclear leukocytes became more stiffened and CD11b expression was higher in patients with sepsis‐induced disseminated intravascular coagulopathy compared to patients without the condition.ConclusionPolymorphonuclear leukocyte deformability correlated with the severity of sepsis‐induced disseminated intravascular coagulopathy and the response to treatment.