Expression Levels of MicroRNA-192 and -215 in Gastric Carcinoma
Expression Levels of MicroRNA-192 and -215 in Gastric Carcinoma
复制标题
胃癌中microRNA-192和-215的表达水平
DOI：
10.1007/s12253-011-9480-x
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发表时间：
2012-07-01
影响因子：
2.8
通讯作者：
Xu, Huimian
中科院分区：
文献类型：
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作者：
Chiang, Yeunpo;Zhou, Xin;Xu, Huimian
微小RNA（miRNAs）是内源性来源的小的非编码RNA。越来越多的研究表明，miRNA的异常表达在多种肿瘤中起重要作用。miR-192和miR-215具有相同的“种子区”，尚未使用胃癌中的大量病例进行全面研究。从118例胃癌组织、3株胃癌细胞系及配对的癌旁组织中提取总RNA。在多聚腺苷酸化和逆转录后，通过实时PCR测定miR-192和-215的表达水平，并使用2（-Delta Delta CT）方法计算，以评价miR-192和-215表达水平与临床病理特征之间的关联。胃癌组织和非肿瘤组织中miR-192和-215的表达水平无显著差异（均p > 0.05，配对t检验）。有趣的是，miR-192和-215在MGC-803细胞、BGC-823细胞和SGC-7901细胞中下调（所有p < 0.01，配对t检验）。此外，miR-192和-215的下调被证明与肿瘤大小增加（均p = 0.003，Mann-Whitney U检验）和晚期Borrmann型肿瘤（分别p = 0.015和p = 0.044，Kruskal-Wallis H检验）相关。此外，miR-192在胃癌pT 4期的表达显著低于pT 1、pT 2和pT 3期（p = 0.026）。此外，胃癌组织中miR-192和-215之间存在强相关性（p < 0.001，Pearson回归）。miR-192和-215可能与胃癌的增殖和侵袭有关。它们有可能成为重要的生物标志物。
MicroRNAs (miRNAs) are small, non-coding RNAs of endogenous origin. Accumulating studies have shown aberrant miRNA expression plays an important role in many tumor types. miR-192 and -215, which have the same "seed region", have not been comprehensively investigated using a large number of cases in gastric cancer. The total RNA was extracted from 118 gastric cancer tissues and three gastric cancer cell lines as well as matched non-tumor adjacent tissues (NATs). After polyadenylation and reverse transcription, expression levels of miR-192 and -215 were determined by real-time PCR and calculation using the 2(-Delta Delta CT) method for evaluation of the association between miR-192, and -215 expression levels and clinicopathological characteristics. There were no significant differences in miR-192 and -215 expression levels between gastric cancer tissues and non-tumor counterparts (both p > 0.05, paired t-test). Interestingly, miR-192 and -215 were down-regulated in MGC-803 cells, BGC-823 cells and SGC-7901 cells (all p < 0.01, paired t-test). Also, the down-regulation of miR-192 and -215 was demonstrated to be associated with increased tumor sizes (both p = 0.003, Mann-Whitney U test) and advanced Borrmann type tumors (p = 0.015 and p = 0.044, respectively, Kruskal-Wallis H test). Moreover, the expression of miR-192 was significantly lower in the pT4 stage of gastric cancer than in pT1, pT2 and pT3 stages (p = 0.026). Furthermore, there was a strong correlation between miR-192 and -215 in gastric cancer tissues (p < 0.001, Pearson regressions). miR-192 and -215 might be related to the proliferation and invasion of gastric cancer. Potentially, they could become important biomarkers.