Bacterial communities in manganese nodules in rice field subsoils: Estimation using PCR-DGGE and sequencing analyses
Bacterial communities in manganese nodules in rice field subsoils: Estimation using PCR-DGGE and sequencing analyses
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DOI：
10.1111/j.1747-0765.2007.00176.x
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发表时间：
2007-10
影响因子：
2
通讯作者：
V. Cahyani;J. Murase;E. Ishibashi;S. Asakawa;M. Kimura
中科院分区：
文献类型：
--
作者：
V. Cahyani;J. Murase;E. Ishibashi;S. Asakawa;M. Kimura
摘要采用聚合酶链式反应-变性梯度凝胶电泳法(PCR-DGGE)结合16S rDNA测序，对两个日本稻田土壤下锰(Mn)根瘤中细菌群落的系统发育位置进行了研究。DGGE谱型和特征DGGE谱带的序列分析表明，锰结核中的细菌群落与耕层和下层土壤中的细菌群落明显不同。在两个地点的锰结核中发现的四条常见条带中，有三条与三角洲蛋白细菌相对应，并被鉴定为硫酸盐还原细菌和铁还原细菌。锰结核的其他DGGE条带分别对应于硫酸盐和铁还原细菌(三角洲蛋白细菌)、甲烷氧化细菌(伽玛和α蛋白细菌)、亚硝酸盐氧化细菌(硝酸螺旋菌)和放线杆菌。此外，一些锰结核的DGGE条带没有显示出与任何已知细菌的明显联系。研究表明，稻田底土中锰结核细菌群落主要由参与锰结核还原的细菌组成。
Abstract The phylogenetic positions of bacterial communities in manganese (Mn) nodules from subsoils of two Japanese rice fields were estimated using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis followed by sequencing of 16S rDNA. The DGGE band patterns and sequencing analysis of characteristic DGGE bands revealed that the bacterial communities in Mn nodules were markedly different from those in the plow layer and subsoils. Three out of four common bands found in Mn nodules from two sites corresponded to Deltaproteobacteria and were characterized as sulfate-reducing and iron-reducing bacteria. The other DGGE bands of Mn nodules corresponded to sulfate and iron reducers (Deltaproteobacteria), methane-oxidizing bacteria (Gamma and Alphaproteobacteria), nitrite-oxidizing bacteria (Nitrospirae) and Actinobacteria. In addition, some DGGE bands of Mn nodules showed no clear affiliation to any known bacteria. The present study indicates that members involved in the reduction of Mn nodules dominate the bacterial communities in Mn nodules in rice field subsoils.