Synthesis, characterization, and preliminary biological study of poly(3-(tert-butoxycarbonyl)-N-vinyl-2-pyrrolidone).
Synthesis, characterization, and preliminary biological study of poly(3-(tert-butoxycarbonyl)-N-vinyl-2-pyrrolidone).
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DOI：
10.1021/bm0256505
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发表时间：
2003
影响因子：
6.2
通讯作者：
W. He;K. Gonsalves;J. H. Pickett;C. Halberstadt
中科院分区：
文献类型：
--
作者：
W. He;K. Gonsalves;J. H. Pickett;C. Halberstadt
合成了聚（3-叔丁氧羰基-N-乙烯基-2-吡咯烷酮），并用凝胶渗透色谱、傅里叶变换红外光谱、核磁共振谱和热分析等手段对其进行了表征。该聚合物是一种化学放大光致抗蚀剂。用这种聚合物通过光刻法成功地图案化了具有25微米宽度和25微米间距的线阵列。将大鼠成纤维细胞接种在这些图案化的表面以及光滑的玻璃表面上。相衬显微镜显示，与在光滑表面上随机铺展相比，图案化表面上的细胞强烈地沿沟槽沿着排列和伸长。由于控制细胞取向对于开发先进形式的组织修复和细胞工程治疗（例如，外周神经修复、体外产生肌腱和韧带替代物以及控制微血管修复）是至关重要的，因此所述聚合物可用于组织重建中的应用。
Poly(3-(tert-butoxycarbonyl)-N-vinyl-2-pyrrolidone) has been synthesized and characterized by gel permeation chromatography, Fourier transform infrared spectroscopy, NMR spectroscopy, and thermal analysis. The polymer is a chemically amplified photoresist. Arrays of lines with 25 microm width and 25 microm spacing were successfully patterned with this polymer by photolithography. Rat fibroblast cells were seeded on these patterned surfaces as well as the smooth glass surface. Phase contrast microscopy showed that cells on the patterned surfaces were strongly aligned and elongated along the grooves as compared to randomly spreading on the smooth surface. Since controlling cell orientation is critical for the development of advanced forms of tissue repair and cell engineering therapies, for example, peripheral nerve repair, production of tendon and ligament substitutes in vitro, and control of microvascular repair, the described polymer may be useful for applications in tissue reconstruction.