Cytokine profiles in cerebrospinal fluid of patients with meningitis at a tertiary general hospital in China
Cytokine profiles in cerebrospinal fluid of patients with meningitis at a tertiary general hospital in China
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中国某三级综合医院脑膜炎患者脑脊液细胞因子谱。
DOI：
10.1016/j.jmii.2018.08.019
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发表时间：
2020-04-01
影响因子：
7.4
通讯作者：
Shao, Lingyun
中科院分区：
文献类型：
--
作者：
Liu, Qianqian;Gao, Yan;Shao, Lingyun
背景：已有大量证据表明细胞因子参与脑膜炎症。中枢神经系统(CNS)感染可能因病原体不同而呈现不同的细胞因子谱。目的：探讨中枢神经系统感染患者脑脊液中细胞因子的变化。方法：选择43例中枢神经系统感染患者，包括结核性脑膜炎、化脓性脑膜炎和隐球菌性脑膜炎，并以11例脑脊液正常者作为对照组。采用多重细胞因子分析法检测脑脊液中Th1、Th2和Th17型细胞因子的浓度。结果：CNS感染患者脑脊液中IL-1β、IL-4、IL-6、IL-10、IL-17、IL-23、IL-33、干扰素-γ、肿瘤坏死因子-α和sCD40L水平均高于对照组(均P<0.05)。中枢神经系统感染患者脑脊液IL-6水平明显升高，与对照组比较重叠程度最小。脑脊液IL-6水平与脑脊液葡萄糖及脑脊液/血糖比值呈显著负相关(r=-0.4375，P=0.0042；r=-0.4991，P=0.0009)。结论：中枢神经系统感染时存在以脑脊液中Th1、Th2和Th17型细胞因子水平升高为特征的免疫反应过度激活。此外，我们还观察到中枢神经系统感染患者的脑脊液IL-6水平与脑脊液血糖及脑脊液/血糖比值呈负相关。我们认为联合检测脑脊液IL-6和脑脊液葡萄糖水平可作为鉴别中枢神经系统感染的新的生物标志物。版权所有(C)2018，台湾微生物学会。爱思唯尔台湾有限责任公司出版。
Background: There has been a great deal of evidence indicating that cytokines participate in meningeal inflammation. Different cytokine profiles may be presented in central nervous system (CNS) infection due to different pathogens. We have attempted to investigate cytokine profiles in cerebrospinal fluid (CSF) of patients with CNS infection.Methods: Forty-three patients with CNS infection including tuberculous meningitis, purulent meningitis and cryptococcal meningitis were enrolled and 11 patients with normal CSF were enrolled as control group. The concentrations of Th1-, Th2- and Th17-type cytokines in CSF were detected using multiplex cytokine assay. Furthermore, the correlation between CSF cytokines and CSF parameters in CNS infection was analyzed.Results: The CSF levels of IL-1 beta, IL-4, IL-6, IL-10, IL-17, IL-23, IL-33, IFN-gamma, TNF-alpha and sCD40L among the patients with CNS infection were all higher than control group (all P < 0.05). A remarkable elevation of CSF IL-6 in the patients with CNS infection was observed with the least overlap of the CSF concentrations compared to controls. Moreover, CSF IL-6 levels were strongly negatively correlated with CSF glucose and the CSF/blood glucose ratio (r = -0.4375, P = 0.0042; r = -0.4991, P = 0.0009).Conclusions: The excessive activation of immune response characterized by elevated levels of CSF Th1-, Th2- and Th17-type cytokines has been observed during CNS infection. Furthermore, we observed negative correlations between CSF IL-6 levels and CSF glucose and CSF/blood glucose ratio in CNS infection. And we suggested that combined CSF IL-6 levels with CSF glucose may serve as a novel biomarker pool for the differential of CNS infection. Copyright (C) 2018, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.