Isolation and identification of arctiin and arctigenin in leaves of burdock (Arctium lappla L.) by polyamide column chromatography in combination with HPLC-ESI/MS
Isolation and identification of arctiin and arctigenin in leaves of burdock (Arctium lappla L.) by polyamide column chromatography in combination with HPLC-ESI/MS
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DOI：
10.1002/pca.816
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发表时间：
2005-03-01
影响因子：
3.3
通讯作者：
Strack, D
中科院分区：
文献类型：
--
作者：
Liu, SM;Chen, KS;Strack, D
采用聚酰胺柱层析- HPLC-PAD和HPLC-ESI/MS相结合的方法分离鉴定牛蒡叶中的木质素素牛蒡素和牛蒡素。用80%甲醇提取牛蒡叶后，将粗提物的水相分为水和氯仿，水相在聚酰胺玻璃柱上进行分馏。该馏分用100%甲醇洗脱，在4℃下浓缩并得到白色沉淀，其中两种主要化合物通过半制备型高效液相色谱纯化。通过紫外光谱、ESI-MS光谱和HPLC保留时间的比较，确定化合物为牛蒡子素和牛蒡子素。采用内标法对提取/分离技术进行验证。版权所有2005 John Wiley A Sons， Ltd。
A simple method involving polyamide column chromatography in combination with HPLC-PAD and HPLC-ESI/MS for isolating and identifying two kinds of lignans, arctiin and arctigenin, in the leaves of burdock (Arctium lappa L.) has been established. After extraction of burdock leaves with 80% methanol, the aqueous phase of crude extracts was partitioned between water and chloroform and the aqueous phase was fractionated on a polyamide glass column. The fraction, eluting with 100% methanol, was concentrated and gave a white precipitate at 4 degrees C from which two main compounds were purified by semi-preparative HPLC. In comparison with the UV and ESI-MS spectra and the HPLC retention time of authentic standards, the compounds were determined to be arctiin and arctigenin. The extraction/separation technique was validated using an internal standard method. Copyright (c) 2005 John Wiley A Sons, Ltd.