Identification and characterization of integron-associated antibiotic resistant Laribacter hongkongensis isolated from aquatic products in China
Identification and characterization of integron-associated antibiotic resistant Laribacter hongkongensis isolated from aquatic products in China
复制标题
中国水产品中整合子相关抗生素耐药香港落叶杆菌的鉴定和表征
DOI：
10.1016/j.ijfoodmicro.2010.10.014
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发表时间：
2011-01-05
影响因子：
5.4
通讯作者：
Chen, Qing
中科院分区：
文献类型：
--
作者：
Feng, Jia-li;Yan, He;Chen, Qing
香港海鸥菌是近年发现的一种与肠胃炎有关的细菌。在这项研究中，共199个分离的水产品（n = 462），在中国广州市，该物种进行了检查，他们对19种抗菌药物的敏感性和抗菌药物耐药整合子的存在。还评估了具有整合子的分离物的遗传相关性。一种基于PCR的方法被用来筛选整合子，发现13（6.5%）的分离物窝藏1类整合子。整合子阳性菌株对头孢吡肟、环丙沙星、庆大霉素、四环素、复方新诺明和利福平的耐药率明显高于整合子阴性菌株。基因序列分析表明，这些整合子含有各种抗菌药物抗性基因（dfrA 1、dfrA 14、dfrA 17、dfrA 32、aadA 1、aadA 2、aadA 5、cmlA 5、arr 2、ereA和orfC），这些基因被组织成不同的基因盒排列，包括一种新的dfrA 14-arr 2-cmlA 5阵列。脉冲场凝胶电泳（PFGE）在13个整合子阳性菌株中产生了13种不同的模式，可分为4个集群。这些表明多重耐药整合子在不同分子类型中的分散。据我们所知，这是第一个报告显示的1类整合子之间的分布和特征的L hongkongensis分离物。(C)2010爱思唯尔有限公司版权所有。
Laribacter hongkongensis is a recently discovered bacterium associated with gastroenteritis. In this study, a total of 199 isolates of this species obtained from aquatic products (n = 462) in Guangzhou City, China, were examined for their susceptibility to 19 antimicrobial agents and the presence of antimicrobial resistance integrons. The genetic relatedness of the isolates with integrons was also evaluated. A PCR-based method was used to screen integrons and found that 13 (6.5%) of the isolates harbored class 1 integrons. The antimicrobial resistance rates of integron-positive isolates were significantly higher than integron-negative ones for cefepime, ciprofloxacin, gentamicin, tetracycline, trimethoprim-sulfamethoxazole and rifampicin. Genetic sequence analysis revealed that these integrons contained various antimicrobial-resistance genes (dfrA1 dfrA14, dfrA17, dfrA32, aadA1, aadA2, aadA5, cmlA5, arr2, ereA and orfC) organized into different gene cassettes arrangements including a novel array of dfrA14-arr2-cmlA5. Pulsed-field gel electrophoresis (PFGE) yielded 13 different patterns among 13 integron-positive isolates, which could be grouped into four clusters. These indicate the dispersal of multi-resistant integrons among different molecular types. To the best of our knowledge, this is the first report showing distribution and characterization of class 1 integrons among L hongkongensis isolates. (C) 2010 Elsevier B.V. All rights reserved.