Oscope identifies oscillatory genes in unsynchronized single-cell RNA-seq experiments.
Oscope identifies oscillatory genes in unsynchronized single-cell RNA-seq experiments.
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DOI：
10.1038/nmeth.3549
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发表时间：
2015-10
期刊：
影响因子：
48
通讯作者：
Kendziorski C
中科院分区：
文献类型：
--
作者：
Leng N;Chu LF;Barry C;Li Y;Choi J;Li X;Jiang P;Stewart RM;Thomson JA;Kendziorski C
振荡基因表达是哺乳动物发育的基础，但监测表达振荡的技术有限。我们开发了一种称为 Oscope 的统计方法，用于识别和表征来自不同步细胞群的单细胞 RNA-seq 数据中振荡基因的转录动态。对多个数据集的应用证明了该方法的实用性，并确定了 Fluidigm C1 平台中的潜在工件。
Oscillatory gene expression is fundamental to mammalian development, but technologies to monitor expression oscillations are limited. We have developed a statistical approach called Oscope to identify and characterize the transcriptional dynamics of oscillating genes in single-cell RNA-seq data from an unsynchronized cell population. Applications to a number of data sets demonstrate the utility of the approach and also identify a potential artifact in the Fluidigm C1 platform.