The growth suppressing gas1 product is a GPI-linked protein
The growth suppressing gas1 product is a GPI-linked protein
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DOI：
10.1016/s0014-5793(00)02004-4
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发表时间：
2000-09-15
期刊：
影响因子：
3.5
通讯作者：
Schneider, C
中科院分区：
文献类型：
--
作者：
Stebel, M;Vatta, P;Schneider, C
Growth arrest specific (gas) 1 gene product is expressed in non-transformed fibroblasts in response to stimuli driving cells into Go phase. Gas1 has been demonstrated to inhibit cell proliferation when over-expressed in proliferating fibroblasts, This activity depends on a function of the p53 protein independent of its transactivating ability. To better define the pathway leading from Gas1, which is located on the plasma membrane, to p53, we have undertaken a detailed characterization of its topology, We demonstrate that the protein undergoes cotranslational modifications in the endoplasmic reticulum, consisting of signal peptide cleavage, N-linked glycosylation and glycosyl-phosphatidylinositol anchor addition, Immunoelectron microscopy shows that, in its mature form, Gas1 is randomly distributed over the outer leaflet of the plasma membrane and that upon antibody-induced clustering it relocalizes to caveolae, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
