In Vitro Investigation of Enhanced Hemocompatibility and Endothelial Cell Proliferation Associated with Quinone-Rich Polydopamine Coating
In Vitro Investigation of Enhanced Hemocompatibility and Endothelial Cell Proliferation Associated with Quinone-Rich Polydopamine Coating
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与富含醌的聚多巴胺涂层相关的增强血液相容性和内皮细胞增殖的体外研究
DOI：
10.1021/am3027635
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发表时间：
2013-03-13
影响因子：
9.5
通讯作者：
Huang, Nan
中科院分区：
文献类型：
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作者：
Luo, Rifang;Tang, Linlin;Huang, Nan
最近的研究表明，聚多巴胺(PDA)修饰的表面有利于内皮细胞(ECs)的增殖。在这项工作中，PDA涂层的316L不锈钢(316L SS)分别在50、100和150℃(以下称为Th50、Th100和Th150)进行热处理，从而产生不同的表面化学成分。体外血液相容性和血管细胞-材料相互作用的内皮细胞和血管内皮细胞(SMCs)的表面特性的影响进行了研究。富含苯二酚的Th150表现出最好的血液相容性，并能有效地抑制血小板黏附、活化和纤维蛋白原构象转变。经多巴胺修饰的表面可诱导细胞-材料相互作用，促进内皮细胞的增殖、活性和迁移，释放一氧化氮(NO)，抑制血管内皮细胞的增殖。抑制SMC增殖的作用可能与其表面儿茶酚含量有关。Th150涂层对血管支架的压缩和扩张变形具有良好的抵抗能力。这些结果有效地表明，Th150涂层作为支架涂层平台可能是很有前途的。
Recent investigations have demonstrated that polydopamine (PDA)-modified surfaces were beneficial to the proliferation of endothelial cells (ECs). In this work, PDA coated 316L stainless steels (316L SS) were thermally treated at 50, 100, and 150 degrees C respectively (hereafter designated as Th50, Th100, and Th150) and consequently produced diverse surface chemical components. In vitro hemocompatibility and vascular cell-material interactions with ECs and smooth muscle cells (SMCs) affected by surface characteristics have been investigated. The Th150, rich in quinone, showed the best hemocompatibility and could effectively inhibit platelet adhesion, activation, and fibrinogen conformation transition. The polydopamine-modified surfaces were found to induce dramatic cell-material interaction with enhanced ECs proliferation, viability and migration, release of nitric oxide (NO), and reduced SMCs proliferation. The inhibitory effect of SMCs proliferation might be associated with the surface catechol content. The coating on Th150 showed a good resistance to the deformation of compression and expansion of vascular stents. These results effectively suggested that the Th150 coating might be promising when served as a stent coating platform.