Relative Prevalence and Antimicrobial Susceptibility of Clinical Isolates of Elizabethkingia Species Based on 16S rRNA Gene Sequencing
Relative Prevalence and Antimicrobial Susceptibility of Clinical Isolates of Elizabethkingia Species Based on 16S rRNA Gene Sequencing
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DOI：
10.1128/jcm.01637-16
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发表时间：
2017-01-01
影响因子：
9.4
通讯作者：
Chong, Yunsop
中科院分区：
文献类型：
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作者：
Han, Mi-Soon;Kim, Hyunsoo;Chong, Yunsop
一些先前报道的伊莉莎白菌脑膜炎败血症临床分离株可能后来被命名为伊莉莎白菌种。采用两种基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）系统和Vitek 2 GN卡，测定了物种鉴定的准确性，临床标本中三种伊丽莎白属的相对流行率，以及16S rRNA基因测序鉴定的物种的抗菌敏感性。培养标本是在2009年至2015年期间从韩国首尔一家大学医院的患者身上收集的。患者中均检出3种伊丽莎白氏菌；经16S rRNA基因测序鉴定的86株分离株中，脑膜炎脓毒杆菌17株（19.8%）、miricola伊丽莎白金氏菌18株（20.9%）、按蚊伊丽莎白金氏菌51株（59.3%）。只有带有修正数据库的MALDI-TOF Vitek MS系统正确地鉴定了所有分离株。大多数分离株（76.7%）来自下呼吸道，8株（9.3%）来自血液。超过90%的脑膜炎脓毒杆菌和按蚊分离株对哌拉西林-他唑巴坦和利福平敏感。除环丙沙星外，其余分枝杆菌均对氟喹诺酮类药物敏感。目前迫切需要进一步的研究来确定治疗每一种elizabeth ethkingia感染的最佳抗菌药物。
Some of the previously reported clinical isolates of Elizabethkingia meningoseptica may be later named species of Elizabethkingia. We determined the accuracy of species identification (with two matrix-assisted laser desorption ionization-time of flight mass spectrometry [MALDI-TOF MS] systems and the Vitek 2 GN card), relative prevalence of three Elizabethkingia spp. in clinical specimens, and antimicrobial susceptibility of the species identified by 16S rRNA gene sequencing. Specimens for culture were collected from patients in a university hospital in Seoul, South Korea, between 2009 and 2015. All 3 Elizabethkingia spp. were detected in patients; among the 86 isolates identified by 16S rRNA gene sequencing, 17 (19.8%) were E. meningoseptica, 18 (20.9%) were Elizabethkingia miricola, and 51 (59.3%) were Elizabethkingia anophelis. Only the MALDI-TOF Vitek MS system with an amended database correctly identified all of the isolates. The majority (76.7%) of the isolates were from the lower respiratory tract, and 8 (9.3%) were from blood. Over 90% of E. meningoseptica and E. anophelis isolates were susceptible to piperacillin-tazobactam and rifampin. In contrast, all E. miricola isolates were susceptible to fluoroquinolones except ciprofloxacin. Further studies are urgently needed to determine the optimal antimicrobial agents for the treatment of infections due to each individual Elizabethkingia species.