FTIR microspectroscopic analysis of normal human cortical and trabecular bone
FTIR microspectroscopic analysis of normal human cortical and trabecular bone
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DOI：
10.1007/s002239900371
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发表时间：
1997-12-01
影响因子：
4.2
通讯作者：
Boskey, AL
中科院分区：
文献类型：
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作者：
Paschalis, EP;Betts, F;Boskey, AL
傅里叶变换红外显微光谱（FTIRM）已被用来研究在非骨质疏松症的人皮质骨和松质骨的重复活检的矿物质和基质的含量和组成的变化。先前报告了这些相同样本中的骨细胞骨的变化。从两例尸检病例的髂嵴活检中获得了沿着和穿过椎板的光谱图。矿物：基质比（分别根据900-1200 cm（-1）处的磷酸盐nu（1）、nu（3）带和大约1585-1725 cm（-1）处的酰胺I带的积分面积计算）在两个分析方向上相对恒定，即，沿着沿着和穿过薄板。结合二阶导数光谱和曲线拟合分析nu（1），nu（3）磷酸盐谱带的组分，发现存在11个主要的潜在部分。其中，在约1020 cm（-1）和约1030 cm（-1）处的两个基本条带的相对面积比已被证明是人类骨细胞骨和人工合成的结晶不良磷灰石中晶体完整性变化的敏感指标。该比值是在沿着和穿过椎板的人髂嵴活检的皮质骨和松质骨中计算的。在皮质骨中，从骨膜到髓腔，以及从骨小梁的周边到中心，该比率降低。这些观察结果在从相同活检获得的连续切片、从相同尸检标本获得的多个活检以及从两个不同尸检标本获得的活检中一致。沿着呈现的结果以及先前报道的骨细胞骨的变化显示了骨龄与“结晶度/成熟度”之间的关系。（该参数取决于微晶尺寸、类羟基磷灰石化学计量、取代离子如CO 32的丰度。结晶/成熟越多，类羟基磷灰石化学计量越多，微晶尺寸越大，如由1020/1030 cm（-1）比率推断的，被离子如CO 32-取代的离子越少。当然，年轻的正常骨比年长的正常骨更不成熟/结晶。这些结果为描述矿物质特性提供了一个“基线”，患病的骨骼可以与之进行比较。
Fourier transform infrared microspectroscopy (FTIRM) has been used to study the changes in mineral and matrix content and composition in replicate biopsies of nonosteoporotic human cortical and trabecular bone. Changes in osteonal bone in these same samples were reported previously. Spectral maps along and across the lamellae were obtained from iliac crest biopsies of two necropsy cases. Mineral:matrix ratios, calculated from the integrated areas of the phosphate nu(1), nu(3) band at 900-1200 cm(-1) and the amide I band at approximate to 1585-1725 cm(-1), respectively, were relatively constant in both directions of analysis, i.e., along and across the lamellae. Analysis of the components of the nu(1), nu(3) phosphate band with a combination of second-derivative spectroscopy and curve fitting revealed the presence of 11 major underlying moieties. Of these, the ratio of the relative areas of the two underlying bands at approximate to 1020 and approximate to 1030 cm(-1) has been shown to be a sensitive index of variation in crystal perfection in both human osteonal bone and in synthetic, poorly crystalline apatites. This ratio was calculated in both cortical and trabecular bone from human iliac crest biopsies along and across the lamellae. The ratio decreased, going from the periosteum to the medullary cavity in the cortical bone, and from the periphery towards the center of trabeculae. These observations were consistent within serial sections obtained from the same biopsy, multiple biopsies obtained from the same necropsy specimen, and biopsies obtained from the two different necropsy specimens. The results presented here along with previously reported changes in osteonal bone show a relation between bone age and ''crystallinity/maturity'' (a parameter dependent on crystallite size, hydroxyapatite-like stoichiometry, abundance of substituting ions such as CO32- the more crystalline/mature, the more hydroxyapatite-like stoichiometry, the bigger the crystallite size, the less the ion substitution by ions such as CO32-) as deduced by the 1020/1030 cm(-1) ratio. Invariably, younger normal bone is less mature/ crystalline than older. These results provide a ''baseline'' for description of mineral properties, to which diseased bones may be compared.