Microbiome dataset from Clara Cave and Empalme Sinkhole waters in Puerto Rico.
Microbiome dataset from Clara Cave and Empalme Sinkhole waters in Puerto Rico.
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来自波多黎各 Clara Cave 和 Empalme Sinkhole 水域的微生物组数据集。
DOI：
10.1016/j.dib.2018.11.028
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发表时间：
2018
期刊：
影响因子：
1.2
通讯作者：
Rios-Velazquez,Carlos
中科院分区：
文献类型：
--
作者：
Rodriguez-Ramos,LuisE;Rios-Velazquez,Carlos
卡穆伊河洞穴公园(CRCP)是一个地下洞穴系统，位于波多黎各北部亚热带潮湿森林中卡穆伊河雕刻的亚热带喀斯特地带(Nieves-Rivera，2003)[1]。本文包含来自Clara Cave和Empalme Sinkhole水样的微生物和功能多样性的元基因组数据集。直接从样品中提取环境DNA(EDNA)，然后采用下一代测序技术(Illumina MiSeq)。这些序列被提交到MG-RAST在线服务器，用于分类图谱的生成和样本的电子描述功能。数据由结构域细菌(96.69%)、病毒(2.87%)、真核生物(0.37%)和古生菌(0.02%)组成。数据按门分布依次为变形杆菌(92.61%)、类杆菌(1.66%)、放线杆菌(1.12%)和革兰氏杆菌(0.48%)。子系统功能数据显示，12.97%的基因与聚类子系统相关，11.40%的基因与碳水化合物相关，11.0%的基因与氨基酸及其衍生物相关。生成的元基因组数据集将提供对洞穴中存在的微生物组成和功能多样性的理解和比较框架。
Camuy River Cave Park (CRCP) is an underground cave system located at the subtropical karst carved by the Camuy River in the subtropical moist forest of northern Puerto Rico (Nieves-Rivera, 2003) [1]. This article contains a metagenomic dataset from the microbial and functional diversity of Clara Cave and Empalme Sinkhole water samples. The environmental DNA (eDNA) from the samples was extracted following direct Metagenomic DNA Isolation method, followed by Next-Generation-Sequencing technology (Illumina MiSeq). The sequences were submitted to MG-RAST online server for taxonomic profile generation and functionalin silicodescription of the samples. The data consisted of domain Bacteria (96.69%), followed up by Viruses (2.87%), Eukaryotes (0.37%), and Archaea (0.02%). The data distribution by phyla showedProteobacteria(92.61%),Bacteroidetes(1.66%),Actinobacteria(1.12%), andFirmicutes(0.48%). The subsystem functional data showed that 12.97% of genes were related to clustering-based subsystems, 11.40% to carbohydrates, and 11.0% to amino acids and derivatives. The metagenome dataset generated will provide an understanding and comparison framework of the microbial composition and functional diversity present in caves.