REQUIREMENT OF AN ADHERENT CELL SUBSTRATUM FOR GROWTH OF DEVELOPING PLASMACYTOMA CELLS INVIVO
REQUIREMENT OF AN ADHERENT CELL SUBSTRATUM FOR GROWTH OF DEVELOPING PLASMACYTOMA CELLS INVIVO
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DOI：
10.1084/jem.144.6.1554
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发表时间：
1976-01-01
影响因子：
15.3
通讯作者：
POTTER, M
中科院分区：
文献类型：
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作者：
CANCRO, M;POTTER, M
腹腔注射降植烷（2，6，10，14-四甲基十五烷）可在短短3天内产生有利于原发性浆细胞瘤生长的环境。在降植烷注射后，总的游离腹膜细胞群从正常值1.55 × 10 - 6增加到正常值1.55 × 10 - 6。106至5.28 ×106，并保持在这个高水平至少50天。由单核细胞和多形核白细胞组成的粘附腹膜细胞群是这种增加的主要来源。在降植烷处理的腹膜中，这些细胞迅速在腹膜结缔组织上形成慢性肉芽肿。每天用0.5 mg氢化可的松皮下处理小鼠，同时注射降植烷，可防止腹膜细胞群的增加、肉芽肿形成和条件环境的产生。在降植烷注射后3天开始用氢化可的松处理的小鼠中，既不能阻止腹膜细胞增加，也不能阻止条件环境的产生。以4天的间隔腹膜内注射巯基乙酸盐培养基产生了与降植烷类似的游离粘附腹膜细胞群的升高，但不产生肉芽肿或条件环境。巯基乙酸诱导的粘附性腹膜细胞的腹膜内转移到用降植烷和氢化可的松处理的小鼠中，同时恢复了条件环境的产生。这些发现表明，粘附腹膜细胞群是负责的调节效果，并建立一个居民人口的这些细胞是必要的，以产生调节。
The i.p. injection of pristane (2,6,10,14-tetramethylpentadecane) produces an environment conducive to primary plasmacytoma growth in as few as 3 days. After pristane injection, the total free peritoneal cell population increases from a normal value of 1.55 .times. 106 to 5.28 .times. 106 and remains at this elevated level for at least 50 days. The adherent peritoneal cell population, composed of mononuclear cells and polymorphonuclear leukocytes, is the primary source of this increase. In the pristane-conditioned peritoneum, these cells rapidly form a chronic granuloma on the peritoneal connective tissues. Daily subcutaneous treatment of mice with 0.5 mg of hydrocortisone beginning simultaneously with pristane injection prevents the increase in the peritoneal cell population, granuloma formation and the production of a conditioned environment. In mice treated with hydrocortisone beginning 3 days after pristane injection, neither the peritoneal cell increase nor the production of a conditioned environment is prevented. The i.p. injection of thioglycolate medium at 4 day intervals produces an elevation of the free adherent peritoneal cell population similar to pristane, but does not produce a granuloma or a conditioned environment. The intraperitoneal transfer of thioglycolate induced adherent peritoneal cells to mice treated with pristane and hydrocortisone simultaneously restores the production of a conditioned environment. These findings indicate that the adherent peritoneal cell population is responsible for the conditioning effect, and the establishment of a resident population of these cells is necessary to produce conditioning.