Signalling pathways leading to transcriptional regulation of genes involved in the activation of glycolysis in yeast
Signalling pathways leading to transcriptional regulation of genes involved in the activation of glycolysis in yeast
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DOI：
10.1046/j.1365-2958.1997.4811847.x
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发表时间：
1997-08-01
影响因子：
3.6
通讯作者：
Planta, RJ
中科院分区：
文献类型：
--
作者：
Goncalves, PM;Griffioen, G;Planta, RJ
向在不太优选的碳源上生长的酵母细胞中添加葡萄糖会引发几种基因表达水平的深刻变化。本文重点介绍了酿酒酵母从呼吸生长向发酵生长转变过程中糖酵解转录激活的信号转导途径。为此，我们研究了糖酵解基因（PFK 1，PYK 1和PDC），一个促凝基因（FBP 1）和两个编码6-磷酸果糖-2-激酶同工酶的基因（PFK 26和PFK 27）在这个转变过程中的转录调控。使用糖酵解突变体、不同的可发酵碳源和2-脱氧葡萄糖的实验结果表明，这些基因的适当转录调节依赖于三种己糖激酶中的任何一种形成葡萄糖6-磷酸的能力。此外，我们的结论是，通过Ras-腺苷酸环化酶途径的信号传导是没有必要的糖酵解和产糖基因对葡萄糖的适当的转录反应，因为这些基因的转录没有显着影响突变体具有高或低的活动，这条途径。与此相反，PFK 26和PFK 27基因的转录调控显着改变在几个Ras-腺苷酸环化酶途径突变体的研究，表明蛋白激酶A在这些基因的转录调控中起着重要作用。
Addition of glucose to yeast cells growing on less preferred carbon sources triggers profound changes in the expression levels of several genes. This paper focuses on the signal transduction pathways leading to transcriptional activation of the glycolysis in Saccharomyces cerevisiae during the transition from respiratory to fermentative growth conditions. To this end, we studied the transcriptional regulation of glycolytic genes (PFK1, PYK1 and PDC), one gluconeogenic gene (FBP1) and the two genes encoding the 6-phosphofructo-2-kinase isoenzymes (PFK26 and PFK27) during this transition. The results of experiments using glycolysis mutants, different fermentable carbon sources and 2-deoxyglucose indicate that proper transcriptional regulation of these genes is dependent on the ability to form glucose 6-phosphate by any one of the three hexose kinases. In addition, we conclude that signalling via the Ras-adenylate cyclase pathway is not necessary for the proper transcriptional response of glycolytic and gluconeogenic genes to glucose, because the transcription of these genes is not significantly affected in mutants having either high or low activities of this pathway. In contrast, the transcriptional regulation of the PFK26 and PFK27 genes is significantly altered in several of the Ras-adenylate cyclase pathway mutants studied, indicating that protein kinase A plays an important role in the transcriptional regulation of these genes.