Mannitol enhances therapeutic effects of intra-arterial transplantation of mesenchymal stem cells into the brain after traumatic brain injury
Mannitol enhances therapeutic effects of intra-arterial transplantation of mesenchymal stem cells into the brain after traumatic brain injury
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DOI：
10.1016/j.neulet.2013.08.058
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发表时间：
2013-10-25
影响因子：
2.5
通讯作者：
Date, Isao
中科院分区：
文献类型：
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作者：
Okuma, Yu;Wang, Feifei;Date, Isao
在交通事故或跌倒中持续的创伤性脑损伤（TBI）是影响广泛年龄范围的死亡的主要原因。本研究的目的是探讨动脉内移植间充质干细胞（MSCs）联合高渗甘油（25%）或甘露醇（25%）在大鼠TBI模型的治疗效果。TBI模型用流体冲击装置制造。在TBI后24小时，通过右颈内动脉给予MSC（1 × 10（6）细胞/100 μ l）和甘油或甘露醇。对大鼠进行行为学和组织化学评价，并探讨高渗溶液诱导的血脑屏障（BBB）通透性增高。与PBS或甘油相比，甘露醇的施用导致BBB破坏增加。甘露醇治疗的大鼠表现出运动功能的显着改善。骨髓间充质干细胞动脉内移植未引起血栓栓塞性缺血。免疫组化结果显示，移植后24 h，甘露醇组大鼠脑组织中的MSCs数量明显多于甘油组和PBS组。骨髓间充质干细胞联合甘露醇动脉内移植是治疗大鼠TBI的有效方法。该技术可用于包括TBI在内的中枢神经系统疾病患者。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Traumatic brain injury (TBI) sustained in a traffic accident or a fall is a major cause of death that affects a broad range of ages. The aim of this study was to investigate the therapeutic effects of intra-arterial transplantation of mesenchymal stem cells (MSCs) combined with hypertonic glycerol (25%) or mannitol (25%) in a TBI model of rats. TBI models were produced with a fluid percussion device. At 24 h after TBI, MSCs (1 x 10(6) cells/100 mu l) with glycerol or mannitol were administered via the right internal carotid artery. Rats were evaluated behaviorally and immunohistochemically, and hyperpermeability of the blood-brain barrier (BBB) induced by hypertonic solutions was explored. Compared to PBS or glycerol, the administration of mannitol resulted in increased BBB disruption. The mannitol-treated rats showed significant improvement in motor function. Intra-arterial transplantation of MSCs caused no thromboembolic ischemia. Immunohistochemically, more MSCs were observed in the injured brain tissues of mannitol-treated rats than in glycerol or PBS-treated rats at 24 h after transplantation. Intra-arterial transplantation of MSCs combined with mannitol is an effective treatment in a TBI model of rats. This technique might be used for patients with diseases of the central nervous system including TBI. (C) 2013 Elsevier Ireland Ltd. All rights reserved.