Red cell metabolism in the newborn infant. V. Glycolytic intermediates and glycolytic enzymes.
Red cell metabolism in the newborn infant. V. Glycolytic intermediates and glycolytic enzymes.
复制标题
新生儿红细胞代谢。
作者：
F. Oski
以往的研究表明，新生儿的红细胞有一个相对的磷酸果糖激酶缺乏和不适当的葡萄糖消耗细胞年龄。鉴于这些发现，在婴儿、正常成人和网织红细胞增多症受试者的红细胞中分析了红细胞糖酵解中间产物和所有Embden-Meyerhof途径酶活性，以进一步确定代谢差异并寻找代谢阻断的证据。
网织红细胞增多症患者的红细胞中葡萄糖-6-磷酸、果糖-6-磷酸和2，3-二磷酸甘油酸的含量增加。早产儿红细胞中磷酸己糖的升高更大，并与2，3-二磷酸甘油酸和磷酸烯醇丙酮酸水平的降低有关。
酶活性的测量表明，除了磷酸果糖激酶缺乏，新生儿的细胞有显着增加的烯醇化酶和磷酸甘油酸激酶的活动。
这表明，在婴儿和网织红细胞增多症患者的红细胞中的磷酸己糖水平的增加是这种相对磷酸果糖激酶缺乏症的反映，这种缺乏症在新生儿中更严重。新生儿红细胞和正常成人红细胞之间酶谱的差异不仅仅是其年龄较小的函数。一些差异反映了这一生命时期特有的细胞的存在。
Previous studies demonstrated that the erythrocytes of the newborn infant have a relative phosphofructokinase deficiency and inappropriate glucose consumption for cell age. In view of these findings, an analysis of red cell glycolytic intermediates and all Embden-Meyerhof pathway enzyme activities was made in the red cells of infants, normal adults, and subjects with reticulocytosis to define further differences in metabolism and search for evidence of a metabolic blockade.
The erythrocytes of subjects with reticulocytosis were found to possess increased quantities of glucose-6-phosphate, fructose-6-phosphate and 2,3-diphosphoglycerate. The elevation of the hexose phosphates was even greater in the red cells of the premature infants and was associated with a decrease in levels of 2,3-diphosphoglycerate and phosphoenolpyruvate.
Measurement of enzyme activities indicated that, in addition to the phosphofructokinase deficiency, the cells of the newborn infant have markedly increased activities of enolase and phosphoglycerate kinase.
It is suggested that the increase in the level of the hexose phosphates in the erythrocytes of the infants and subjects with reticulocytosis is a reflection of this relative phosphofructokinase deficiency, with this deficiency being greater in newborn infants. The differences in the enzymatic profile between the erythrocytes of the newborn and those of the normal adult is not solely a function of their younger age. Some of the differences are a reflection of the presence of a cell unique to this period of life.