Rapid prototyping of 3D DNA-origami shapes with caDNAno.
Rapid prototyping of 3D DNA-origami shapes with caDNAno.
复制标题
DOI：
10.1093/nar/gkp436
复制
发表时间：
2009-08
影响因子：
14.9
通讯作者：
Shih WM
中科院分区：
文献类型：
--
作者：
Douglas SM;Marblestone AH;Teerapittayanon S;Vazquez A;Church GM;Shih WM
DNA纳米技术利用基本配对提供的可编程特异性，以产生自定义形状的大型组合物，用于构建Megadalton-Scale-Scale-Scale DNA纳米结构，可用于模板，以模板为数百个寡核纤维纤维'Staple'Staple'Staple'Staple'Staple'''链中的一个平面式螺旋线阵列，我们最近改编了这种“脚手架DNA折纸”方法以双螺旋的折叠层被束缚在蜂窝状晶格上，但是，完成所需的设计步骤可能是繁琐的且耗时的。折叠3D蜂窝状形状的DNA序列设计，组装和分析了一系列矩形块图案用于未来的研究，Cadnano的使用显着减少了设计3D DNA-ORIGAMI结构所需的努力代码是根据MIT许可发布的。
DNA nanotechnology exploits the programmable specificity afforded by base-pairing to produce self-assembling macromolecular objects of custom shape. For building megadalton-scale DNA nanostructures, a long ‘scaffold’ strand can be employed to template the assembly of hundreds of oligonucleotide ‘staple’ strands into a planar antiparallel array of cross-linked helices. We recently adapted this ‘scaffolded DNA origami’ method to producing 3D shapes formed as pleated layers of double helices constrained to a honeycomb lattice. However, completing the required design steps can be cumbersome and time-consuming. Here we present caDNAno, an open-source software package with a graphical user interface that aids in the design of DNA sequences for folding 3D honeycomb-pleated shapes A series of rectangular-block motifs were designed, assembled, and analyzed to identify a well-behaved motif that could serve as a building block for future studies. The use of caDNAno significantly reduces the effort required to design 3D DNA-origami structures. The software is available at http://cadnano.org/, along with example designs and video tutorials demonstrating their construction. The source code is released under the MIT license.