Difference in response to aluminum stress among Tibetan wild barley genotypes
Difference in response to aluminum stress among Tibetan wild barley genotypes
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西藏野生大麦基因型对铝胁迫响应的差异
DOI：
10.1002/jpln.201100148
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发表时间：
2011-12-01
影响因子：
2.5
通讯作者：
Wu, Feibo
中科院分区：
文献类型：
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作者：
Dai, Huaxin;Shan, Wunian;Wu, Feibo
野生大麦（Hordeum sp.）种质资源具有丰富的遗传多样性，为作物改良提供了有用的基因宝库。我们利用105个一年生西藏野生大麦和45个栽培大麦基因型，进行了一项综合计划，将通过根再生程序的苏木素染色进行的短期水培筛选和基于滤纸的评估不同种质对铝/酸胁迫的响应结合起来。105个西藏野生大麦基因型根系伸长量在铝胁迫下差异显著，变异系数为62.9%~ 80.0%，多样性指数为4.35 ~ 4.45。这些基因型差异铝抗性是相当一致的，在这两个水培和滤纸为基础的评价：XZ 16，XZ 166，和XZ 113被选为铝抗性基因型，和XZ 61，XZ 45，和XZ 98为铝敏感的野生型。此外，在滤纸上的实验中，检测到的根和地上部中的铝浓度显着低于3个敏感基因型中的3个抗铝基因型。同时，在长期水培试验中，XZ 16在降低SPAD值（叶绿素计读数）、株高、根长、干生物量、单株分蘖数和叶绿素荧光（Fv/Fm）等方面受铝毒影响最小。代顿的应激症状最严重，而XZ 61的应激症状最严重。
Wild barley (Hordeum sp.) germplasm is rich in genetic diversity and provides a treasure trove of useful genes for crop improvement. We carried out a comprehensive program combining short-term hydroponic screening via hematoxylin-staining of root-regrowth procedure and filter paper–based evaluation of diverse germplasm in response to Al/acid stress using 105 annual Tibetan wild barley and 45 cultivated barley genotypes. Root elongation among the 105 Tibetan wild barley genotypes varied significantly after Al exposure, ranging from 62.9% to 80.0% in variation coefficients and 4.35 to 4.45 in diversity index. These genotypic differences in Al resistance were fairly consistent in both the hydroponic and filter paper–based evaluations: XZ16, XZ166, and XZ113 were selected as Al-resistant genotypes, and XZ61, XZ45, and XZ98 as Al-sensitive wild genotypes. Furthermore, significantly lower Al concentrations in roots and shoots were detected in the three selected Al-resistant genotypes than in the three sensitive genotypes in the filter paper–based experiment. Meanwhile, XZ16 was the least affected by Al toxicity in regard to reduced SPAD value (chlorophyll meter readings), plant height, root length, dry biomass, tillers per plant, and chlorophyll fluorescence (Fv/Fm) in the long-term hydroponic experiment compared with the Al-resistant cultivated barley cv. Dayton, while XZ61 had the severest stress symptoms.