Ionic currents and endogenous rhythm generation in the pre-Bötzinger complex: modelling and in vitro studies.
Ionic currents and endogenous rhythm generation in the pre-Bötzinger complex: modelling and in vitro studies.
复制标题
前 Bötzinger 复合体中的离子电流和内源节律产生：建模和体外研究。
DOI：
10.1007/0-387-27023-x_19
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发表时间：
2004
影响因子：
--
通讯作者：
Rybak,IlyaA
中科院分区：
文献类型：
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作者：
Pierrefiche,Olivier;Shevtsova,NataliaA;St-John,WalterM;Paton,JulianFR;Rybak,IlyaA
前Bötzinger复合体（pBC）是延髓头端腹外侧的一个小区域，被认为是哺乳动物呼吸网络的"核心" 1 - 5。在某些实验条件下，含有该区域的新生啮齿动物的体外制剂可以产生内在的节律活动4，5。这种活动不需要抑制性神经传递6，因此，很可能是由pBC 1 - 5中的起搏神经元群体产生的。同时，体外记录的pBC节律活动的"递减"放电模式不同于体内正常条件下观察到的呼吸放电模式（"正常呼吸"），并且类似于喘息模式7 - 8。为了建立pBC内在节律活动与体内呼吸节律发生的可能关系，重要的是分析这种活动在体外发生的条件，并将这些条件与体内正常呼吸和喘息期间的节律发生条件进行比较。根据初步建模研究9，pBC中的体外节律活动可能依赖于pBC神经元中电压门控钾电流和持续钠电流的相对表达。在这里，我们提出了我们的联合建模和体外研究的结果，以测试这种建模预测。我们的研究集中在钾电流和持续性钠电流参与体外培养的PBC的内源性节律性活动以及这种活动与体内呼吸振荡发生的可能关系。
The pre-Bötzinger complex (pBC), a small area in the rostroventrolateral medulla, has been suggested to represent a “kernel” of the mammalian respiratory network1–5. Thein vitropreparations from neonatal rodents containing this area can, under certain experimental conditions, generate an intrinsic rhythmic activity4,5. This activity does not require inhibitory neurotransmission6 and, therefore, is likely to be generated by a population of pacemaker neurons in the pBC1–5. At the same time, the “decrementing” discharge pattern of rhythmic activity in the pBC recordedin vitrodiffers from the pattern of respiratory discharges observed under normal conditionsin vivo(“eupnoea”) and is similar to gasping pattern7–8. In order to establish possible relationships of the intrinsic rhythmic activity in the pBC to the respiratory rhythmogenesisin vivo, it is important to analyse the conditions in which this activity occursin vitroand to compare these conditions with the rhythmogenic conditions during eupnoea and gaspingin vivo. According to the preliminary modelling studies9, thein vitrorhythmic activity in the pBC may be dependent on a relative expression of the voltage-gated potassium and persistent sodium currents in pBC neurons. Here we present the results of our combined modelling andin vitrostudies performed to test this modelling prediction. Our studies focused on the involvement of the potassium and persistent sodium currents in the endogenous rhythmic activity in the pBCin vitroand on the possible relation of this activity to the genesis of the respiratory oscillationsin vivo.