Depletion of autophagy receptor p62/SQSTM1 enhances the efficiency of gene delivery in mammalian cells
Depletion of autophagy receptor p62/SQSTM1 enhances the efficiency of gene delivery in mammalian cells
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DOI：
10.1002/1873-3468.12262
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发表时间：
2016-08-01
期刊：
影响因子：
3.5
通讯作者：
Haraguchi, Tokuko
中科院分区：
文献类型：
--
作者：
Tsuchiya, Megumi;Ogawa, Hidesato;Haraguchi, Tokuko
提高基因传递到细胞的效率的新方法将具有许多有用的应用。在这里，我们报告了一个简单的方法，涉及耗尽p62/SQSTM 1，以提高基因传递的效率。p62基因敲除小鼠胚胎成纤维细胞（MEF）的报告基因的效率显着高于正常MEF细胞相比。这种更高的效率被部分衰减的异位表达p62。此外，siRNA介导的p62敲低明显增加了小鼠胚胎干细胞（mES）和人HeLa细胞的转染效率。这些数据表明，p62作为基因传递的关键调节因子。
Novel methods that increase the efficiency of gene delivery to cells will have many useful applications. Here, we report a simple approach involving depletion of p62/SQSTM1 to enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was remarkably higher in p62-knockout murine embryonic fibroblast (MEF) cells compared with normal MEF cells. This higher efficiency was partially attenuated by ectopic expression of p62. Furthermore, siRNA-mediated knockdown of p62 clearly increased the efficiency of transfection of murine embryonic stem (mES) cells and human HeLa cells. These data indicate that p62 acts as a key regulator of gene delivery.