IFI27 Is a Useful Genetic Marker for Diagnosis of Immunoglobulin A Nephropathy and Membranous Nephropathy Using Peripheral Blood.
IFI27 Is a Useful Genetic Marker for Diagnosis of Immunoglobulin A Nephropathy and Membranous Nephropathy Using Peripheral Blood.
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DOI：
10.1371/journal.pone.0153252
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Nojima H
中科院分区：
文献类型：
--
作者：
Nagasawa Y;Okuzaki D;Muso E;Yamamoto R;Shinzawa M;Iwasaki Y;Iwatani H;Nakanishi T;Isaka Y;Nojima H
慢性肾小球肾炎（CGN）的诊断主要依靠肾活检，费用昂贵且需要住院治疗，因此需要对该疾病的无创诊断方法。我们使用 DNA 微阵列分析来寻找外周血单核细胞 (PBMC) 中表达水平可以区分 CGN 患者和健康志愿者 (HV) 的基因。我们选择免疫球蛋白 A 肾病 (IgAN) 和膜性肾病 (MN) 作为 CGN 的典型形式。编码干扰素 (IFN)-α 诱导蛋白 27 (IFI27) 的基因的 mRNA 水平在 IgAN 和 MN 患者的 PBMC 中低于 HV 患者，该基因优先在肾小球足细胞中表达。该结果通过定量实时逆转录聚合酶链反应（qRT-PCR）得到证实。此外，qRT-PCR 分析显示，其他类型慢性肾小球肾炎患者的 PBMC 中 IFI27 mRNA 水平降低。活检标本的 IFI27 免疫组织化学染色也证实 IgAN 和 MN 患者中 IFI27 蛋白的表达降低。基于这些结果，我们建议 IFI27 可以作为使用外周血的 CGN 的无创诊断标记物。
Diagnosis of chronic glomerulonephritis (CGN) depends primarily on renal biopsy, which is expensive and requires hospitalization, creating a demand for noninvasive diagnostic method for this disease. We used DNA microarray analysis to search for genes whose expression levels in peripheral blood mononuclear cells (PBMCs) could distinguish between patients with CGN and healthy volunteers (HVs). We selected immunoglobulin A nephropathy (IgAN) and membranous nephropathy (MN) as typical forms of CGN. The mRNA level of the gene encoding interferon (IFN)-alpha-inducible protein 27, IFI27, which is preferentially expressed in podocytes of glomeruli, was lower in PBMCs of IgAN and MN patients than in those of HVs. This result was confirmed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Moreover, qRT-PCR analysis revealed that the IFI27 mRNA level was reduced in PBMCs of patients with other types of chronic glomerulonephritis. IFI27 immunohistochemical staining of biopsied specimens also confirmed reduced expression of IFI27 protein in IgAN and MN patients. Based on these results, we propose that IFI27 could serve as a noninvasive diagnostic marker for CGNs using peripheral blood.