A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains
A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains
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DOI：
10.1073/pnas.97.26.14668
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发表时间：
2000-12-19
影响因子：
11.1
通讯作者：
Falkow, S
中科院分区：
文献类型：
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作者：
Salama, N;Guillemin, K;Falkow, S
幽门螺杆菌在世界上一半人口的胃中定植，引起广泛的疾病，从无症状的胃炎到溃疡到胃癌。尽管这些不同临床结果的基础尚不清楚，但更严重的疾病与携带致病岛的菌株相关。为了表征强毒株和弱毒株的遗传多样性，我们检测了15 H. pylori临床分离株进行全基因组测序。pylori基因芯片。我们发现，22%的H。pylori基因在一个或多个菌株中是同源的，因此定义了1281 H的最小功能核心。pylori基因。虽然核心基因编码大多数代谢和细胞过程，但菌株特异性基因包括H.幽门螺杆菌，限制性修饰基因，转座酶和编码细胞表面蛋白的基因，这些基因在细菌长期感染遗传多样性宿主期间的特定情况下可能有助于细菌。我们观察到菌株特异性基因沿着染色体沿着分布的不同模式，这可能是由于基因获得和丢失的不同机制造成的。在菌株特异性基因中，我们发现了一类与致病岛共遗传的候选毒力基因。
Helicobacter pylori colonizes the stomach of half of the world's population, causing a wide spectrum of disease ranging from asymptomatic gastritis to ulcers to gastric cancer. Although the basis for these diverse clinical outcomes is not understood, more severe disease is associated with strains harboring a pathogenicity island. To characterize the genetic diversity of more and less virulent strains, we examined the genomic content of 15 H. pylori clinical isolates by using a whole genome H. pylori DNA microarray. We found that a full 22% of H. pylori genes are dispensable in one or more strains, thus defining a minimal functional core of 1281 H. pylori genes. While the core genes encode most metabolic and cellular processes, the strain-specific genes include genes unique to H. pylori, restriction modification genes, transposases, and genes encoding cell surface proteins, which may aid the bacteria under specific circumstances during their long-term infection of genetically diverse hosts. We observed distinct patterns of the strain-specific gene distribution along the chromosome, which may result from different mechanisms of gene acquisition and loss. Among the strain-specific genes, we have found a class of candidate virulence genes identified by their coinheritance with the pathogenicity island.